Interleukin 22 Receptor Alpha 2 (IL22Ra2) ELISA Kit | MyBioSource MBS2022491 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Interleukin 22 Receptor Alpha 2 (IL22Ra2) ELISA Kit

catalog :

MBS2022491

quantity :

24-Strip-Wells

price :

270 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2022491

products type :

ELISA Kit

products full name :

Interleukin 22 Receptor Alpha 2 (IL22Ra2) ELISA Kit

products short name :

[Interleukin 22 Receptor Alpha 2 (IL22Ra2)]

products name syn :

[CRF2-S1; IL-22BP; ZcytoR16; Cytokine receptor class-II member 10; Cytokine receptor family type 2, soluble 1; Interleukin-22-binding protein]

other names :

[interleukin 22 receptor, alpha 2, isoform CRA_a; Interleukin-22 receptor subunit alpha-2; interleukin-22 receptor subunit alpha-2; interleukin 22 receptor, alpha 2; Cytokine receptor class-II member 10; Cytokine receptor family 2 member 10; CRF2-10; Cytokine receptor family type 2, soluble 1; CRF2-S1; Interleukin-22-binding protein; IL-22BP; IL22BP; ZcytoR16]

products gene name :

[IL22Ra2]

other gene names :

[IL22RA2; IL22RA2; CRF2X; CRF2-10; CRF2-S1; IL-22BP; IL-22RA2; ZCYTOR16; IL-22R-alpha-2; IL-22 receptor subunit alpha-2; IL-22R-alpha-2; IL-22RA2; CRF2-10; CRF2-S1; IL-22BP; IL22BP]

uniprot entry name :

I22R2_HUMAN

reactivity :

Mouse

sequence length :

130

specificity :

This assay has high sensitivity and excellent specificity for detection of IL2. No significant cross-reactivity or interference between IL2 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Simian Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.6-1,000pg/mL. Sensitivity: 5.5pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Infection immunity

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL2 in simian serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to IL2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

119568318

ncbi acc num :

EAW47933.1

uniprot acc num :

Q969J5

ncbi mol weight :

15,128 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488)

ncbi summary :

This gene encodes a member of the class II cytokine receptor family. The encoded soluble protein specifically binds to and inhibits interleukin 22 activity by blocking the interaction of interleukin 22 with its cell surface receptor. The encoded protein may be important in the regulation of inflammatory response, and has been implicated in the regulation of tumorigenesis in the colon. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2013]

uniprot summary :

IL22RA2: Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses. Belongs to the type II cytokine receptor family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 6q23.3. Cellular Component: extracellular space; integral to membrane; plasma membrane; cytosol. Molecular Function: interleukin-22 binding; interleukin-22 receptor activity. Biological Process: negative regulation of inflammatory response; regulation of tyrosine phosphorylation of Stat3 protein; cytokine and chemokine mediated signaling pathway

size1 :

24-Strip-Wells

price1 :

270 USD

size2 :

48-Strip-Wells

price2 :

505

size3 :

96-Strip-Wells

price3 :

685

size4 :

5x96-Strip-Wells

price4 :

2770

size5 :

10x96-Strip-Wells

price5 :

5025

more info or order :

MyBioSource product webpage