Paralemmin (PALM) ELISA Kit | MyBioSource MBS2022434 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Paralemmin (PALM) ELISA Kit

catalog :

MBS2022434

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2022434

products type :

ELISA Kit

products full name :

Paralemmin (PALM) ELISA Kit

products short name :

Paralemmin (PALM)

other names :

paralemmin; Paralemmin-1; Paralemmin

products gene name :

PALM

other gene names :

PALM; KIAA0270

uniprot entry name :

PALM_HUMAN

reactivity :

Human

sequence length :

343

specificity :

This assay has high sensitivity and excellent specificity for detection of paralemmin. No significant cross-reactivity or interference between paralemmin and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Human tissue homogenates, Cell lysates and other biological fluids. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of paralemmin is typically less than 0.057ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level paralemmin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level paralemmin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of paralemmin in human tissue homogenates, cell lysates and other biological fluids.

products description :

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to paralemmin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to paralemmin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain paralemmin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of paralemmin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4456613

ncbi acc num :

CAB37400.1

uniprot acc num :

O75781

ncbi mol weight :

37,157 Da

uniprot summary :

paralemmin: Involved in plasma membrane dynamics and cell process formation. Isoform 1 and isoform 2 are necessary for axonal and dendritic filopodia induction, for dendritic spine maturation and synapse formation in a palmitoylation-dependent manner. Belongs to the paralemmin family. 2 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: nucleoplasm; filopodium membrane; intracellular membrane-bound organelle; apicolateral plasma membrane; axon; basolateral plasma membrane; integral to plasma membrane; cytoplasmic membrane-bound vesicle; plasma membrane; nucleus. Molecular Function: protein binding; D3 dopamine receptor binding. Biological Process: positive regulation of filopodium formation; regulation of cell shape; synapse maturation; negative regulation of dopamine receptor signaling pathway; negative regulation of adenylate cyclase activity; cytoskeleton organization and biogenesis; cell motility

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage