ELISA/assay

Deiodinase, Iodothyronine, Type II (DIO2) ELISA Kit

MBS2022432

48-Strip-Wells

565 USD

ELISA Kit

Deiodinase, Iodothyronine, Type II (DIO2) ELISA Kit

Deiodinase, Iodothyronine, Type II (DIO2)

5DII; D2; SelY; TXDI2; Thyroxine Deiodinase,Type II; Type-II 5'-deiodinase

Deiodinase, iodothyronine, type II; Type II iodothyronine deiodinase; type II iodothyronine deiodinase; deiodinase, iodothyronine, type II; 5DII; DIOII; Type 2 DI; Type-II 5'-deiodinase

DIO2

DIO2; DIO2; D2; 5DII; SelY; DIOII; TXDI2; ITDI2; TXDI2

IOD2_HUMAN

Human

309

This assay has high sensitivity and excellent specificity for detection of Deiodinase, Iodothyronine, Type II (DIO2). No significant cross-reactivity or interference between Deiodinase, Iodothyronine, Type II (DIO2) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.055ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Deiodinase, Iodothyronine, Type II (DIO2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Deiodinase, Iodothyronine, Type II (DIO2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deiodinase, Iodothyronine, Type II (DIO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deiodinase, Iodothyronine, Type II (DIO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deiodinase, Iodothyronine, Type II (DIO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Deiodinase, Iodothyronine, Type II (DIO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

223462734

AAI36515.1

Q92813

34,704 Da

Amine-derived Hormones Pathway (160983); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Regulation Of Thyroid Hormone Activity Pathway (160986); Selenium Metabolism And Selenoproteins Pathway (198846); Selenium Pathway (198825); Thyroid Hormone Signaling Pathway (946598); Thyroxine Biosynthesis Pathway (160985); Thyroid Hormone Metabolism I (via Deiodination) Pathway (545360); Thyroid Hormone Metabolism I (via Deiodination) Pathway (139512)

The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It is highly expressed in the thyroid, and may contribute significantly to the relative increase in thyroidal T3 production in patients with Graves disease and thyroid adenomas. This protein contains selenocysteine (Sec) residues encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

DIO2: Responsible for the deiodination of T4 (3,5,3 ,5 - tetraiodothyronine) into T3 (3,5,3 -triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development. Belongs to the iodothyronine deiodinase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; EC 1.97.1.10; Oxidoreductase. Chromosomal Location of Human Ortholog: 14q24.2-q24.3. Cellular Component: membrane; plasma membrane; integral to membrane. Molecular Function: selenium binding; thyroxine 5 -deiodinase activity; ubiquitin protein ligase binding. Biological Process: thyroid hormone generation; hormone biosynthetic process; selenocysteine incorporation; thyroid hormone metabolic process

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340