ELISA/assay

V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) ELISA Kit

MBS2022354

96 Strip Wells

745 USD

ELISA Kit

V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) ELISA Kit

V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB)

REL-B; I-REL; Transcription Factor RelB; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3

V-rel reticuloendotheliosis viral oncogene homolog B; Transcription factor RelB; transcription factor RelB; v-rel avian reticuloendotheliosis viral oncogene homolog B; I-Rel

RELB

RELB; RELB; IREL; I-REL; REL-B

RELB_HUMAN

Human

579

This assay has high sensitivity and excellent specificity for detection of V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB). No significant cross-reactivity or interference between V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Sample Types: Tissue homogenates and other biological fluids. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of this kit is typically less than 0.063ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

20380825

AAH28013.1

Q01201

62,134 Da

Alternative NF-kappaB Pathway 138004!!Epstein-Barr Virus Infection Pathway 585562!!Epstein-Barr Virus Infection Pathway 587115!!HTLV-I Infection Pathway 373901!!HTLV-I Infection Pathway 373889!!IL12-mediated Signaling Events Pathway 137922!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!NF-kappa B Signaling Pathway 634527!!Osteoclast Differentiation Pathway 193147

96 Strip Wells

745 USD