ELISA/assay

Mouse Phosphatidylserine (PS) ELISA Kit

MBS2022199

48-Strip-Wells

515 USD

ELISA Kit

Mouse Phosphatidylserine (PS) ELISA Kit

Phosphatidylserine (PS)

phosphatidylserine; phosphatidylserine

PS

Spaf_2022

Mouse

529

This assay has high sensitivity and excellent specificity for detection of PS. No significant cross-reactivity or interference between PS and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Mouse tissue homogenates, Cell lysates and other biological fluids. Assay Type: Competitive. Detection Range: 123.5-10,000pg/mL. Sensitivity: The minimum detectable dose of PS is typically less than 45.6pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by subtracting two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PS were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PS were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of PS in mouse tissue homogenates, cell lysates and other biological fluids.

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PS has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PS and unlabeled PS (Standards or samples) with the pre-coated antibody specific to PS. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PS in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PS in the sample.

386793925

AFJ26960.1

Glycerophospholipid Metabolism Pathway (654016); Glycerophospholipid Metabolism Pathway (364); Metabolic Pathways (654043)

48-Strip-Wells

515 USD

96-Strip-Wells

705

5x96-Strip-Wells

2760

10x96-Strip-Wells

4985