ELISA/assay

Bcl2 Like Protein 11 (BCL2L11) ELISA Kit

MBS2022196

96 Strip Wells

845 USD

ELISA Kit

Bcl2 Like Protein 11 (BCL2L11) ELISA Kit

Bcl2 Like Protein 11 (BCL2L11)

BOD; BimL; BimEL; BIM; Bcl2-interacting mediator of cell death

bcl-2-like protein 11 isoform 11; Bcl-2-like protein 11; bcl-2-like protein 11; BCL2-like 11 (apoptosis facilitator); Bcl2-interacting mediator of cell death

BCL2L11

BCL2L11; BCL2L11; BAM; BIM; BOD; BIM; Bcl2-L-11

B2L11_HUMAN

Human

73

This assay has high sensitivity and excellent specificity for detection of Bcl2 Like Protein 11 (BCL2L11). No significant cross-reactivity or interference between Bcl2 Like Protein 11 (BCL2L11) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Sample Types: Tissue homogenates, cell lysates and other biological fluids. Detection Range: 0.313-20ng/mL. Sensitivity: The minimum detectable dose of this kit is typically less than 0.117ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2 Like Protein 11 (BCL2L11) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2 Like Protein 11 (BCL2L11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Like Protein 11 (BCL2L11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Like Protein 11 (BCL2L11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Like Protein 11 (BCL2L11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Bcl2 Like Protein 11 (BCL2L11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

323362959

NP_001191035.1

NM_001204106.1

O43521

8,523 Da

Activation Of BH3-only Proteins Pathway 105658!!Activation Of BIM And Translocation To Mitochondria Pathway 105662!!Apoptosis Pathway 198797!!Apoptosis Pathway 105648!!Apoptosis Modulation And Signaling Pathway 198822!!B Cell Receptor Signaling Pathway 198909!!BDNF Signaling Pathway 712093!!BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway 105664!!Cell Death Signalling Via NRAGE, NRIF And NADE Pathway 106443!!DNA Damage Response (only ATM Dependent) Pathway 198827

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013]

96 Strip Wells

845 USD