Alpha-1-Acid Glycoprotein (a1AGP) ELISA Kit | MyBioSource MBS2022190 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Alpha-1-Acid Glycoprotein (a1AGP) ELISA Kit

catalog :

MBS2022190

quantity :

48-Strip-Wells

price :

525 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2022190

products type :

ELISA Kit

products full name :

Alpha-1-Acid Glycoprotein (a1AGP) ELISA Kit

products short name :

Alpha-1-Acid Glycoprotein (a1AGP)

products name syn :

AGP1; a1-AGP; ORM1; OMD 1; Orosomucoid 1

other names :

alpha-1 acid glycoprotein; Alpha-1-acid glycoprotein 1; Orosomucoid-1; OMD 1

products gene name :

a1AGP

other gene names :

ORM1; AGP1; AGP 1; OMD 1

uniprot entry name :

A1AG1_HUMAN

reactivity :

Mouse

sequence length :

201

specificity :

This assay has high sensitivity and excellent specificity for detection of Alpha-1-Acid Glycoprotein (a1AGP). No significant cross-reactivity or interference between Alpha-1-Acid Glycoprotein (a1AGP) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 62.5-4000ng/mL. Sensitivity: Typically less than 25.3ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-1-Acid Glycoprotein (a1AGP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-1-Acid Glycoprotein (a1AGP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Acid Glycoprotein (a1AGP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Acid Glycoprotein (a1AGP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Acid Glycoprotein (a1AGP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Alpha-1-Acid Glycoprotein (a1AGP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

178257

ncbi acc num :

AAA35515.1

ncbi mol weight :

23,512 Da

uniprot summary :

ORM1: Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction. Belongs to the calycin superfamily. Lipocalin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9q32. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding. Biological Process: regulation of immune system process; transport; acute-phase response; inflammatory response

size1 :

48-Strip-Wells

price1 :

525 USD

size2 :

96-Strip-Wells

price2 :

715

size3 :

5x96-Strip-Wells

price3 :

2760

size4 :

10x96-Strip-Wells

price4 :

4985

more info or order :

MyBioSource product webpage