ELISA/assay

AE Binding Protein 1 (AEBP1) ELISA Kit

MBS2022177

48-Strip-Wells

565 USD

ELISA Kit

AE Binding Protein 1 (AEBP1) ELISA Kit

AE Binding Protein 1 (AEBP1)

ACLP; Aortic Carboxypeptidase-Like Protein; Adipocyte Enhancer Binding Protein 1

AE binding protein 1; Adipocyte enhancer-binding protein 1; adipocyte enhancer-binding protein 1; AE binding protein 1; Aortic carboxypeptidase-like protein

AEBP1

AEBP1; AEBP1; ACLP; ACLP; AE-binding protein 1

AEBP1_HUMAN

Human

733

This assay has high sensitivity and excellent specificity for detection of AE Binding Protein 1 (AEBP1). No significant cross-reactivity or interference between AE Binding Protein 1 (AEBP1) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/mL. Sensitivity: Typically less than 0.29ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AE Binding Protein 1 (AEBP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AE Binding Protein 1 (AEBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AE Binding Protein 1 (AEBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to AE Binding Protein 1 (AEBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AE Binding Protein 1 (AEBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of AE Binding Protein 1 (AEBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119581523

EAW61119.1

Q8IUX7

82,183 Da

This gene encodes a member of carboxypeptidase A protein family. The encoded protein may function as a transcriptional repressor and play a role in adipogenesis and smooth muscle cell differentiation. Studies in mice suggest that this gene functions in wound healing and abdominal wall development. Overexpression of this gene is associated with glioblastoma. [provided by RefSeq, May 2013]

AEBP1: May positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation. May also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I- kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness. Can act as a transcriptional repressor. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription factor; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7p13. Cellular Component: extracellular matrix; extracellular space; cytoplasm; nucleus. Molecular Function: calmodulin binding; carboxypeptidase activity; zinc ion binding; transcription factor activity; metallocarboxypeptidase activity; transcription corepressor activity. Biological Process: muscle development; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; proteolysis; skeletal development

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340