ELISA/assay

Inhibin Beta E (INHbE) ELISA Kit

MBS2022173

48-Strip-Wells

515 USD

ELISA Kit

Inhibin Beta E (INHbE) ELISA Kit

Inhibin Beta E (INHbE)

INH-bE; Activin beta-E chain

inhibin beta E chain; Inhibin beta E chain; inhibin beta E chain; inhibin, beta E; Activin beta-E chain

INHbE

INHBE; INHBE

INHBE_HUMAN

Human

350

This assay has high sensitivity and excellent specificity for detection of Inhibin Beta E (INHbE). No significant cross-reactivity or interference between Inhibin Beta E (INHbE) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: Typically less than 5.7pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inhibin Beta E (INHbE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inhibin Beta E (INHbE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibin Beta E (INHbE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inhibin Beta E (INHbE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inhibin Beta E (INHbE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Inhibin Beta E (INHbE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

13899338

NP_113667.1

NM_031479.3

P58166

38,561 Da

Activin Signaling Pathway (1084757); Activin Signaling Pathway (1108220); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Glycoprotein Hormones Pathway (160989); Metabolism Of Proteins Pathway (106230); Peptide Hormone Biosynthesis Pathway (160988); Peptide Hormone Metabolism Pathway (771603); Signaling Pathways Regulating Pluripotency Of Stem Cells (1026136); Signaling Pathways Regulating Pluripotency Of Stem Cells (1033502)

INHBE is a member of the activin beta family (see INHBA; MIM 147290) that plays a role in pancreatic exocrine cell growth and proliferation (Hashimoto et al., 2006 [PubMed 16426570]).[supplied by OMIM, May 2008]

INHBE: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 12q13.3. Cellular Component: extracellular space. Molecular Function: growth factor activity; hormone activity; cytokine activity; transforming growth factor beta receptor binding. Biological Process: regulation of apoptosis; regulation of MAPKKK cascade; cell development; growth

48-Strip-Wells

515 USD

96-Strip-Wells

695

5x96-Strip-Wells

2685

10x96-Strip-Wells

4850