ELISA/assay

Serotonin Transporter (SERT) ELISA Kit

MBS2022165

48-Strip-Wells

575 USD

ELISA Kit

Serotonin Transporter (SERT) ELISA Kit

Serotonin Transporter (SERT)

SLC6A4; SLC6-A4; HTT; 5-HTT; 5HTT; OCD1; hSERT; Solute Carrier Family 6,Member 4; Neurotransmitter Transporter,Serotonin; Sodium-dependent serotonin transporter

serotonin transporter; Sodium-dependent serotonin transporter; sodium-dependent serotonin transporter; solute carrier family 6 (neurotransmitter transporter), member 4; 5HT transporter; 5HTT; Solute carrier family 6 member 4

SERT

SLC6A4; SLC6A4; HTT; 5HTT; OCD1; SERT; 5-HTT; SERT1; hSERT; 5-HTTLPR; HTT; SERT; 5HTT

SC6A4_HUMAN

Mouse

672

This assay has high sensitivity and excellent specificity for detection of Serotonin Transporter (SERT). No significant cross-reactivity or interference between Serotonin Transporter (SERT) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.057ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serotonin Transporter (SERT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serotonin Transporter (SERT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serotonin Transporter (SERT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serotonin Transporter (SERT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serotonin Transporter (SERT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Serotonin Transporter (SERT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

58700442

AAW80933.1

P31645

74,979 Da

Monoamine Transport Pathway (198851); SIDS Susceptibility Pathways (198901); Serotonergic Synapse Pathway (525336); Serotonin Transporter Activity Pathway (920962); Synaptic Vesicle Pathway (672457)

This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, and depression-susceptibility in people experiencing emotional trauma. [provided by RefSeq, Jul 2008]

SERT: Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter. Chromosomal Location of Human Ortholog: 17q11.2. Cellular Component: neuron projection; integral to plasma membrane; plasma membrane; endomembrane system; endosome membrane; cytosol; lipid raft. Molecular Function: actin filament binding; serotonin transmembrane transporter activity; protein binding; protein homodimerization activity; myosin binding; serotonin:sodium symporter activity; cocaine binding; syntaxin-1 binding; monoamine transmembrane transporter activity; nitric-oxide synthase binding; Rab GTPase binding. Biological Process: circadian rhythm; response to drug; vasoconstriction; response to toxin; monoamine transport; positive regulation of cell cycle; thalamus development; social behavior; serotonin uptake; negative regulation of organ growth; negative regulation of synaptic transmission, dopaminergic; memory; protein oligomerization; response to estradiol stimulus; negative regulation of granule cell precursor proliferation; negative regulation of neuron differentiation; brain morphogenesis; response to hypoxia; sperm ejaculation; serotonin transport; transmembrane transport; protein homooligomerization; response to nutrient. Disease: Obsessive-compulsive Disorder; Anxiety

48-Strip-Wells

575 USD

96-Strip-Wells

785

5x96-Strip-Wells

3055

10x96-Strip-Wells

5495