ELISA/assay

Adrenocorticotropic Hormone (ACTH) ELISA Kit

MBS2022156

24-Strip-Wells

250 USD

ELISA Kit

Adrenocorticotropic Hormone (ACTH) ELISA Kit

[Adrenocorticotropic Hormone (ACTH)]

[Corticotropin; Adrenocorticotropin]

[adrenocorticotropic hormone, partial; Pro-opiomelanocortin; pro-opiomelanocortin; pro-opiomelanocortin-alpha; Corticotropin-lipotropin]

[ACTH]

[Pomc; Pomc; BE; Npp; ACTH; Clip; Pomc1; Pomc-1; Beta-LPH; alphaMSH; beta-MSH; Gamma-LPH; alpha-MSH; gamma-MSH; Pomc1; POMC; ACTH; CLIP]

COLI_MOUSE

Rat

235

This assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH). No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Standard Curve/Testing Data

Assay Type: Competitive Inhibition. Samples: Serum, Plasma and Other Biological Fluids. Detection Range: 1.23-100pg/mL. Sensitivity: < 0.67pg/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Endocrinology; Hormone metabolism

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adrenocorticotropic Hormone (ACTH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adrenocorticotropic Hormone (ACTH) and unlabeled Adrenocorticotropic Hormone (ACTH) (Standards or samples) with the pre-coated antibody specific to Adrenocorticotropic Hormone (ACTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample.

191659

AAA37169.1

26,707 Da

Adipocytokine Signaling Pathway (83290); Adipocytokine Signaling Pathway (505); Androgen Biosynthesis Pathway (1110882); Biological Oxidations Pathway (1111015); Class A/1 (Rhodopsin-like Receptors) Pathway (1110499); Cytochrome P450 - Arranged By Substrate Type Pathway (1111017); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1111486); Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway (1111462); Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway (1111463); Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway (1111464)

This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing. Processing yields several biologically active peptides, which are involved in diverse cellular functions, such as energy homeostasis, steroidogenesis, and increased melanin production in melanocytes. In mouse deficiency of this gene is associated with obesity, defects in adrenal development, and altered pigmentation. A pseudogene of this gene is located on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

POMC: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Belongs to the POMC family. Protein type: Secreted, signal peptide; Secreted. Cellular Component: peroxisomal matrix; extracellular space; cytoplasm; extracellular region; secretory granule. Molecular Function: type 3 melanocortin receptor binding; G-protein-coupled receptor binding; type 4 melanocortin receptor binding; hormone activity; receptor binding. Biological Process: generation of precursor metabolites and energy; cell-cell signaling; neuropeptide signaling pathway; regulation of blood pressure; regulation of appetite; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; glucose homeostasis; signal transduction; cellular pigmentation

24-Strip-Wells

250 USD

48-Strip-Wells

445

96-Strip-Wells

595

5x96-Strip-Wells

2390

10x96-Strip-Wells

4305