Metadherin (MTDH) ELISA Kit | MyBioSource MBS2022146 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Metadherin (MTDH) ELISA Kit

catalog :

MBS2022146

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2022146

products type :

ELISA Kit

products full name :

Metadherin (MTDH) ELISA Kit

products short name :

Metadherin (MTDH)

products name syn :

3D3; AEG1; LYRIC; Astrocyte elevated gene-1 protein; Lysine-rich CEACAM1 co-isolated protein; Metastasis adhesion protein

other names :

Metadherin; Protein LYRIC; protein LYRIC; metadherin; 3D3/LYRIC; Astrocyte elevated gene-1 protein; AEG-1; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein

products gene name :

MTDH

other gene names :

MTDH; MTDH; 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3; AEG1; LYRIC; AEG-1

uniprot entry name :

LYRIC_HUMAN

reactivity :

Human

sequence length :

582

specificity :

This assay has high sensitivity and excellent specificity for detection of Metadherin (MTDH). No significant cross-reactivity or interference between Metadherin (MTDH) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.059ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Metadherin (MTDH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Metadherin (MTDH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Metadherin (MTDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Metadherin (MTDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Metadherin (MTDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Metadherin (MTDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

28277147

ncbi acc num :

AAH45642.1

uniprot acc num :

Q86UE4

ncbi mol weight :

63,837 Da

ncbi pathways :

Gastric Cancer Network 2 Pathway (760637); Validated Targets Of C-MYC Transcriptional Activation Pathway (169351)

uniprot summary :

Lyric: a protein of unknown function. Protein type: Membrane protein, integral; Nucleolus; Cell adhesion. Chromosomal Location of Human Ortholog: 8q22.1. Cellular Component: nuclear body; intercellular canaliculus; endoplasmic reticulum membrane; nuclear membrane; tight junction; endoplasmic reticulum; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; nucleolus; integral to membrane; nucleus. Molecular Function: NF-kappaB binding; protein binding; double-stranded RNA binding; transcription coactivator activity. Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; lipopolysaccharide-mediated signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of autophagy; negative regulation of apoptosis; activation of NF-kappaB transcription factor

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage