Histatin 1 (HTN1) ELISA Kit | MyBioSource MBS2022124 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Histatin 1 (HTN1) ELISA Kit

catalog :

MBS2022124

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2022124

products type :

ELISA Kit

products full name :

Histatin 1 (HTN1) ELISA Kit

products short name :

Histatin 1 (HTN1)

products name syn :

PPB; HIS1; Histidine-rich protein 1; Post-PB protein;

other names :

histatin-1; Histatin-1; histatin-1; histatin 1; Histidine-rich protein 1; Post-PB protein; PPB

products gene name :

HTN1

other gene names :

HTN1; HTN1; HIS1; HIS1; PPB; His1 31/57; His1 12/38

uniprot entry name :

HIS1_HUMAN

reactivity :

Human

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of Histatin 1 (HTN1). No significant cross-reactivity or interference between Histatin 1 (HTN1) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Saliva, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: Typically less than 0.59ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histatin 1 (HTN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histatin 1 (HTN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histatin 1 (HTN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histatin 1 (HTN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histatin 1 (HTN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Histatin 1 (HTN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4504529

ncbi acc num :

NP_002150.1

ncbi gb acc num :

NM_002159.3

uniprot acc num :

P15515

ncbi mol weight :

6,963 Da

ncbi pathways :

Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352)

ncbi summary :

This gene encodes a member of the histatin family of small, histidine-rich, cationic proteins. They function as antimicrobial peptides and are important components of the innate immune system. Histatins are found in saliva and exhibit antibacterial, antifungal activities and function in wound healing. [provided by RefSeq, Aug 2014]

uniprot summary :

HTN1: Histatins are salivary proteins that are considered to be major precursors of the protective proteinaceous structure on tooth surfaces (enamel pellicle). In addition, histatins exhibit antibacterial and antifungal activities. Belongs to the histatin/statherin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q13. Cellular Component: extracellular region. Molecular Function: protein binding. Biological Process: biomineral formation; killing of cells of another organism; defense response to bacterium; defense response to fungus

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage