ELISA/assay

Thiopurine Methyltransferase (TPMT) ELISA Kit

MBS2022106

48-Strip-Wells

565 USD

ELISA Kit

Thiopurine Methyltransferase (TPMT) ELISA Kit

Thiopurine Methyltransferase (TPMT)

Thiopurine S Methyltransferase

thiopurine methyltransferase; Thiopurine S-methyltransferase; thiopurine S-methyltransferase; thiopurine S-methyltransferase; Thiopurine methyltransferase

TPMT

TPMT; TPMT

TPMT_HUMAN

Human

245

This assay has high sensitivity and excellent specificity for detection of Thiopurine Methyltransferase (TPMT). No significant cross-reactivity or interference between Thiopurine Methyltransferase (TPMT) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: Typically less than 0.65ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thiopurine Methyltransferase (TPMT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thiopurine Methyltransferase (TPMT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thiopurine Methyltransferase (TPMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thiopurine Methyltransferase (TPMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thiopurine Methyltransferase (TPMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Thiopurine Methyltransferase (TPMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

386420

AAB27277.1

P51580

28,180 Da

Biological Oxidations Pathway (105698); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1127639); Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway (1127658); Defective GGT1 Causes Glutathionuria (GLUTH) Pathway (1127659); Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway (1127660); Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway (1127663); Defective OPLAH Causes 5-oxoprolinase Deficiency (OPLAHD) Pathway (1127661); Defective SLC35D1 Causes Schneckenbecken Dysplasia (SCHBCKD) Pathway (1127665); Defective TPMT Causes Thiopurine S-methyltransferase Deficiency (TPMT Deficiency) Pathway (1127666); Defective UGT1A1 Causes Hyperbilirubinemia Pathway (1127667)

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014]

TPMT: Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. Defects in TPMT are the cause of thiopurine S- methyltransferase deficiency (TPMT deficiency). TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus. Belongs to the methyltransferase superfamily. TPMT family. Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Methyltransferase; EC 2.1.1.67. Chromosomal Location of Human Ortholog: 6p22.3. Cellular Component: cytosol. Molecular Function: thiopurine S-methyltransferase activity. Biological Process: methylation; xenobiotic metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process. Disease: Thiopurine S-methyltransferase Deficiency

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340