Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit | MyBioSource MBS2022102 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit

catalog :

MBS2022102

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2022102

products type :

ELISA Kit

products full name :

Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit

products short name :

Triggering Receptor Expressed On Myeloid Cells 2 (TREM2)

products name syn :

Trem2a; Trem2b; Trem2c; Triggering receptor expressed on monocytes 2

other names :

triggering receptor expressed on myeloid cells 2 isoform 1; Triggering receptor expressed on myeloid cells 2; triggering receptor expressed on myeloid cells 2; triggering receptor expressed on myeloid cells 2; Triggering receptor expressed on monocytes 2

products gene name :

TREM2

other gene names :

TREM2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c; TREM-2

uniprot entry name :

TREM2_HUMAN

reactivity :

Human

sequence length :

222

specificity :

This assay has high sensitivity and excellent specificity for detection of Triggering Receptor Expressed On Myeloid Cells 2 (TREM2). No significant cross-reactivity or interference between Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: Typically less than 25.5pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Triggering Receptor Expressed On Myeloid Cells 2 (TREM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Triggering Receptor Expressed On Myeloid Cells 2 (TREM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Triggering Receptor Expressed On Myeloid Cells 2 (TREM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

9507203

ncbi acc num :

NP_061838.1

ncbi gb acc num :

NM_018965.3

uniprot acc num :

Q9NZC2

ncbi mol weight :

24,669 Da

ncbi pathways :

Axon Guidance Pathway (105688); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Developmental Biology Pathway (477129); Immune System Pathway (106386); Innate Immune System Pathway (106387); Osteoclast Differentiation Pathway (193147); Osteoclast Differentiation Pathway (193096); Other Semaphorin Interactions Pathway (119526); Semaphorin Interactions Pathway (119519)

ncbi summary :

This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]

uniprot summary :

TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.1. Cellular Component: intracellular membrane-bound organelle; plasma membrane; extracellular region; integral to membrane. Molecular Function: peptidoglycan binding; lipopolysaccharide binding; receptor activity. Biological Process: axon guidance; positive regulation of peptidyl-tyrosine phosphorylation; detection of lipopolysaccharide; detection of peptidoglycan; innate immune response; positive regulation of calcium-mediated signaling; humoral immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II. Disease: Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage