Vascular Endothelial Growth Factor 121 (VEGF121) ELISA Kit | MyBioSource MBS2022082 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Vascular Endothelial Growth Factor 121 (VEGF121) ELISA Kit

catalog :

MBS2022082

quantity :

24-Strip-Wells

price :

220 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2022082

products type :

ELISA Kit

products full name :

Vascular Endothelial Growth Factor 121 (VEGF121) ELISA Kit

products short name :

[Vascular Endothelial Growth Factor 121 (VEGF121)]

other names :

[vascular endothelial growth factor isoform 121; Vascular endothelial growth factor A; vascular endothelial growth factor A; vascular endothelial growth factor A; Vascular permeability factor; VPF]

products gene name :

[VEGF121]

other gene names :

[VEGFA; VEGFA; VPF; VEGF; MVCD1; VEGF; VEGF-A; VPF]

uniprot entry name :

VEGFA_HUMAN

reactivity :

Human

sequence length :

317

specificity :

This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor 121 (VEGF121). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor 121 (VEGF121) and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 15.6-1000pg/mL. Sensitivity: < 6.7pg/mL

other info2 :

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor 121 (VEGF121) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor 121 (VEGF121) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

products categories :

Cytokine; Signal transduction; Tumor immunity; Infection immunity

products description :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor 121 (VEGF121). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor 121 (VEGF121). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Factor 121 (VEGF121), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Vascular Endothelial Growth Factor 121 (VEGF121) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

6631029

ncbi acc num :

AAF19659.1

ncbi mol weight :

34,406 Da

ncbi pathways :

Angiogenesis Pathway (198772); Axon Guidance Pathway (105688); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Cellular Response To Hypoxia Pathway (645259); Cellular Responses To Stress Pathway (645258); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (477129); EPH-Ephrin Signaling Pathway (1127691)

ncbi summary :

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

size1 :

24-Strip-Wells

price1 :

220 USD

size2 :

48-Strip-Wells

price2 :

345

size3 :

96-Strip-Wells

price3 :

460

size4 :

5x96-Strip-Wells

price4 :

1815

size5 :

10x96-Strip-Wells

price5 :

3265

more info or order :

MyBioSource product webpage