ELISA/assay

Cross Linked C-Telopeptide Of Type II Collagen (CTXII) ELISA Kit

MBS2022068

24-Strip-Wells

255 USD

ELISA Kit

Cross Linked C-Telopeptide Of Type II Collagen (CTXII) ELISA Kit

[Cross Linked C-Telopeptide Of Type II Collagen (CTXII)]

[CTX-II; CTX2]

[CTXII]

Human

This assay has high sensitivity and excellent specificity for detection of Cross Linked C-Telopeptide Of Type II Collagen (CTXII). No significant cross-reactivity or interference between Cross Linked C-Telopeptide Of Type II Collagen (CTXII) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Competitive Inhibition. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 123.5-10,000pg/mL. Sensitivity: < 52.3pg/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type II Collagen (CTXII) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type II Collagen (CTXII) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Metabolic pathway; Bone metabolism; Rheumatology

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cross Linked C-Telopeptide Of Type II Collagen (CTXII) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cross Linked C-Telopeptide Of Type II Collagen (CTXII) and unlabeled Cross Linked C-Telopeptide Of Type II Collagen (CTXII) (Standards or samples) with the pre-coated antibody specific to Cross Linked C-Telopeptide Of Type II Collagen (CTXII). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) in the sample.

24-Strip-Wells

255 USD

48-Strip-Wells

455

96-Strip-Wells

610

5x96-Strip-Wells

2470

10x96-Strip-Wells

4445