Involucrin (iNV) ELISA Kit | MyBioSource MBS2022067 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Involucrin (iNV) ELISA Kit

catalog :

MBS2022067

quantity :

48-Strip-Wells

price :

550 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2022067

products type :

ELISA Kit

products full name :

Involucrin (iNV) ELISA Kit

products short name :

Involucrin (iNV)

products name syn :

IVL

other names :

involucrin; Involucrin; involucrin; involucrin

products gene name :

iNV

other gene names :

IVL; IVL

uniprot entry name :

INVO_HUMAN

reactivity :

Mouse

sequence length :

585

specificity :

This assay has high sensitivity and excellent specificity for detection of Involucrin (iNV). No significant cross-reactivity or interference between Involucrin (iNV) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.055ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Involucrin (iNV) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Involucrin (iNV) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Involucrin (iNV). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Involucrin (iNV). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Involucrin (iNV), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Involucrin (iNV) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

44890059

ncbi acc num :

NP_005538.2

ncbi gb acc num :

NM_005547.2

uniprot acc num :

P07476

ncbi mol weight :

68,479 Da

ncbi pathways :

Corticotropin-releasing Hormone Pathway (920957); Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway (169349)

ncbi summary :

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]

uniprot summary :

IVL: Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: cytoplasm; cornified envelope. Molecular Function: protein binding, bridging; structural molecule activity. Biological Process: keratinocyte differentiation; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; keratinization; peptide cross-linking; response to UV-B

size1 :

48-Strip-Wells

price1 :

550 USD

size2 :

96-Strip-Wells

price2 :

750

size3 :

5x96-Strip-Wells

price3 :

2910

size4 :

10x96-Strip-Wells

price4 :

5220

more info or order :

MyBioSource product webpage