Alanine Aminotransferase (ALT) ELISA Kit | MyBioSource MBS2022063 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Alanine Aminotransferase (ALT) ELISA Kit

catalog :

MBS2022063

quantity :

24-Strip-Wells

price :

260 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2022063

products type :

ELISA Kit

products full name :

Alanine Aminotransferase (ALT) ELISA Kit

products short name :

[Alanine Aminotransferase (ALT)]

products name syn :

[GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1]

other names :

[alanine aminotransferase, partial; Alanine aminotransferase 1; alanine aminotransferase 1; glutamic-pyruvate transaminase (alanine aminotransferase); Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1]

products gene name :

[ALT]

other gene names :

[GPT; GPT; AAT1; ALT1; GPT1; AAT1; GPT1; ALT1; GPT 1]

uniprot entry name :

ALAT1_HUMAN

reactivity :

Mouse

sequence length :

496

specificity :

This assay has high sensitivity and excellent specificity for detection of ALT. No significant cross-reactivity or interference between ALT and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.58ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ALT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ALT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Enzyme Infection immunity; Hepatology

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ALT in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ALT. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ALT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ALT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ALT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

1507680

ncbi acc num :

BAA01186.1

uniprot acc num :

P24298

ncbi mol weight :

54,637 Da

ncbi pathways :

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (106173); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567)

ncbi summary :

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]

uniprot summary :

GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. Protein type: EC 2.6.1.2; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: cytosol. Molecular Function: alanine transaminase activity; pyridoxal phosphate binding. Biological Process: amino acid biosynthetic process; L-alanine catabolic process

size1 :

24-Strip-Wells

price1 :

260 USD

size2 :

48-Strip-Wells

price2 :

465

size3 :

96-Strip-Wells

price3 :

625

size4 :

5x96-Strip-Wells

price4 :

2530

size5 :

10x96-Strip-Wells

price5 :

4565

more info or order :

MyBioSource product webpage