ELISA/assay

Mannose Receptor C Type 2 (MRC2) ELISA Kit

MBS2022011

48-Strip-Wells

525 USD

ELISA Kit

Mannose Receptor C Type 2 (MRC2) ELISA Kit

Mannose Receptor C Type 2 (MRC2)

CD280; ENDO180; CLEC13E; UPARAP; C-type lectin domain family 13 member E; Endocytic receptor 180; Macrophage mannose receptor 2; Urokinase receptor-associated protein

mannose receptor, C type 2, isoform CRA_a; C-type mannose receptor 2; C-type mannose receptor 2; mannose receptor, C type 2; C-type lectin domain family 13 member E; Endocytic receptor 180; Macrophage mannose receptor 2; Urokinase-type plasminogen activator receptor-associated protein; UPAR-associated protein; Urokinase receptor-associated protein; CD_antigen: CD280

MRC2

MRC2; MRC2; CD280; UPARAP; CLEC13E; ENDO180; CLEC13E; ENDO180; KIAA0709; UPARAP; UPAR-associated protein; Urokinase receptor-associated protein

MRC2_HUMAN

Mouse

1,479

This assay has high sensitivity and excellent specificity for detection of Mannose Receptor C Type 2 (MRC2). No significant cross-reactivity or interference between Mannose Receptor C Type 2 (MRC2) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: Typically less than 6.5pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Receptor C Type 2 (MRC2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Receptor C Type 2 (MRC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Receptor C Type 2 (MRC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Receptor C Type 2 (MRC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Receptor C Type 2 (MRC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mannose Receptor C Type 2 (MRC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119614748

EAW94342.1

166,674 Da

Adaptive Immune System Pathway (366160); Antigen Processing-Cross Presentation Pathway (477122); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cross-presentation Of Soluble Exogenous Antigens (endosomes) Pathway (477126); Immune System Pathway (106386); Phagosome Pathway (153910); Phagosome Pathway (153859); Tuberculosis Pathway (213780); Tuberculosis Pathway (213743)

This gene encodes a member of the mannose receptor family of proteins that contain a fibronectin type II domain and multiple C-type lectin-like domains. The encoded protein plays a role in extracellular matrix remodeling by mediating the internalization and lysosomal degradation of collagen ligands. Expression of this gene may play a role in the tumorigenesis and metastasis of several malignancies including breast cancer, gliomas and metastatic bone disease. [provided by RefSeq, Feb 2012]

MRC2: May play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix co-operating with the matrix metalloproteinases (MMPs). Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 17q23.2. Cellular Component: focal adhesion; membrane; integral to membrane. Molecular Function: collagen binding; protein binding; carbohydrate binding. Biological Process: osteoblast differentiation; collagen catabolic process; endocytosis

48-Strip-Wells

525 USD

96-Strip-Wells

715

5x96-Strip-Wells

2760

10x96-Strip-Wells

4985