catalog number :
MBS2021984
products type :
ELISA Kit
products full name :
Mucin 2 (MUC2) ELISA Kit
products short name :
[Mucin 2 (MUC2)]
products name syn :
[MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal]
other names :
[mucin 2; Mucin 2; Mucin 2]
products gene name :
[MUC2]
uniprot entry name :
L7XFH1_CHICK
specificity :
This assay has high sensitivity and excellent specificity for detection of Mucin 2 (MUC2). No significant cross-reactivity or interference between Mucin 2 (MUC2) and analogues was observed.
storage stability :
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Assay Type: Double-antibody Sandwich. Samples: Tissue homogenates, Cell lysates and Other Biological Fluids. Detection Range: 1.56-100ng/mL. Sensitivity: < 0.57ng/mL
other info2 :
Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 2 (MUC2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 2 (MUC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%
products categories :
Tumor immunity; Infection immunity; Gastroenterology
products description :
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 2 (MUC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 2 (MUC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 2 (MUC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mucin 2 (MUC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
AGD81026.1
ncbi mol weight :
388,031 Da
size5 :
10x96-Strip-Wells