ELISA/assay

Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) ELISA Kit

MBS2021982

48-Strip-Wells

525 USD

ELISA Kit

Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) ELISA Kit

Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14)

CD258; TR2; HVEML; LIGHT; LTg; Herpes virus entry mediator ligand; Herpesvirus entry mediator ligand

tumor necrosis factor ligand superfamily member 14 isoform 1; Tumor necrosis factor ligand superfamily member 14; tumor necrosis factor ligand superfamily member 14; tumor necrosis factor (ligand) superfamily, member 14; Herpes virus entry mediator ligand; HVEM-L; Herpesvirus entry mediator ligand

TNFSF14

TNFSF14; TNFSF14; LTg; TR2; CD258; HVEML; LIGHT; HVEML; LIGHT; HVEM-L; Herpesvirus entry mediator ligand

TNF14_HUMAN

Mouse

204

This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14). No significant cross-reactivity or interference between Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: Typically less than 5.8pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Tumor Necrosis Factor Ligand Superfamily, Member 14 (TNFSF14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

25952144

NP_003798.2

NM_003807.3

O43557

22,395 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); NF-kappa B Signaling Pathway (634527)

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

TNFSF14: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Inhibitor; Cytokine. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: extracellular space; cytoplasm; integral to membrane; plasma membrane. Molecular Function: protein binding; caspase inhibitor activity; cytokine activity; tumor necrosis factor receptor binding; receptor binding. Biological Process: T cell proliferation; release of cytoplasmic sequestered NF-kappaB; T cell activation; apoptosis; T cell costimulation; immune response; T cell homeostasis; negative regulation of caspase activity; signal transduction; positive regulation of myoblast differentiation

48-Strip-Wells

525 USD

96-Strip-Wells

715

5x96-Strip-Wells

2760

10x96-Strip-Wells

4985