Olfactomedin 4 (OLFM4) ELISA Kit | MyBioSource MBS2021973 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Olfactomedin 4 (OLFM4) ELISA Kit

catalog :

MBS2021973

quantity :

48-Strip-Wells

price :

515 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2021973

products type :

ELISA Kit

products full name :

Olfactomedin 4 (OLFM4) ELISA Kit

products short name :

Olfactomedin 4 (OLFM4)

products name syn :

OLM4; hGC1; hOLfD; OlfD; GW112; GC1; G-CSF-stimulated clone 1 protein; Antiapoptotic protein GW112

other names :

olfactomedin-4; Olfactomedin-4; olfactomedin-4; olfactomedin 4; Antiapoptotic protein GW112; G-CSF-stimulated clone 1 protein; hGC-1; hOLfD

products gene name :

OLFM4

other gene names :

OLFM4; OLFM4; GC1; OLM4; OlfD; GW112; hGC-1; hOLfD; UNQ362; bA209J19.1; GW112; OLM4; hGC-1

uniprot entry name :

OLFM4_HUMAN

reactivity :

Human

sequence length :

510

specificity :

This assay has high sensitivity and excellent specificity for detection of Olfactomedin 4 (OLFM4). No significant cross-reactivity or interference between Olfactomedin 4 (OLFM4) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Typically less than 12.8pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Olfactomedin 4 (OLFM4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Olfactomedin 4 (OLFM4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Olfactomedin 4 (OLFM4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Olfactomedin 4 (OLFM4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Olfactomedin 4 (OLFM4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Olfactomedin 4 (OLFM4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

32313593

ncbi acc num :

NP_006409.3

ncbi gb acc num :

NM_006418.4

uniprot acc num :

Q6UX06

ncbi mol weight :

57,280 Da

ncbi summary :

This gene was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion. [provided by RefSeq, Mar 2011]

uniprot summary :

OLFM4: May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 13q14.3. Cellular Component: extracellular space; specific granule; azurophil granule; mitochondrion; perinuclear region of cytoplasm; plasma membrane; secretory granule. Molecular Function: protein homodimerization activity; cadherin binding; catalytic activity. Biological Process: regulation of apoptosis; metabolic process; negative regulation of immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; cell adhesion; regulation of phagocytosis; protein homooligomerization

size1 :

48-Strip-Wells

price1 :

515 USD

size2 :

96-Strip-Wells

price2 :

695

size3 :

5x96-Strip-Wells

price3 :

2685

size4 :

10x96-Strip-Wells

price4 :

4850

more info or order :

MyBioSource product webpage