ELISA/assay

Arginase II (Arg2) ELISA Kit

MBS2021960

48-Strip-Wells

565 USD

ELISA Kit

Arginase II (Arg2) ELISA Kit

Arginase II (Arg2)

Kidney-type arginase; Non-hepatic arginase

arginase II; Arginase-2, mitochondrial; arginase-2, mitochondrial; arginase 2; Kidney-type arginase; Non-hepatic arginase; Type II arginase

Arg2

ARG2; ARG2

ARGI2_HUMAN

Human

354

This assay has high sensitivity and excellent specificity for detection of Arg2. No significant cross-reactivity or interference between Arg2 and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 3.12-200ng/mL. The standard curve concentrations used for the ELISA's were 200ng/mL, 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.12ng/mL. Sensitivity: 1.41ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arg2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arg2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Arg2 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arg2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arg2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arg2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Arg2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

1763758

AAB39855.1

P78540

38,578 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Polyamine Biosynthesis, Arginine = Ornithine = Putrescine Pathway (413403)

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq, Jul 2008]

ARG2: May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders. Belongs to the arginase family. Protein type: Mitochondrial; EC 3.5.3.1; Hydrolase; Amino Acid Metabolism - arginine and proline. Chromosomal Location of Human Ortholog: 14q24.1. Cellular Component: mitochondrion; mitochondrial matrix. Molecular Function: metal ion binding; arginase activity. Biological Process: striated muscle contraction; arginine metabolic process; ureteric bud development; nitric oxide biosynthetic process; urea cycle

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340