Follicle Stimulating Hormone (FSH) ELISA Kit | MyBioSource MBS2021901 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Follicle Stimulating Hormone (FSH) ELISA Kit

catalog :

MBS2021901

quantity :

24-Strip-Wells

price :

235 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2021901

products type :

ELISA Kit

products full name :

Follicle Stimulating Hormone (FSH) ELISA Kit

products short name :

[Follicle Stimulating Hormone (FSH)]

other names :

[follicle-stimulating hormone; Follitropin subunit beta; follitropin subunit beta; follicle stimulating hormone, beta polypeptide; Follicle-stimulating hormone beta subunit; FSH-B; FSH-beta; Follitropin beta chain]

products gene name :

[FSH]

other gene names :

[FSHB; FSHB; HH24; FSH-B; FSH-beta]

uniprot entry name :

FSHB_HUMAN

reactivity :

Rat

sequence length :

129

specificity :

This assay has high sensitivity and excellent specificity for detection of FSH. No significant cross-reactivity or interference between FSH and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Quantitative Competitive. Detection Range: 2.47-200ng/mL. Sensitivity: <1.11ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FSH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FSH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Endocrinology; Reproductive science; Genetic science; Hormone metabolism

products description :

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of FSH in rat serum, plasma and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to FSH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled FSH and unlabeled FSH (Standards or samples) with the pre-coated antibody specific to FSH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of FSH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of FSH in the sample.

ncbi gi num :

182762

ncbi acc num :

AAA52476.1

uniprot acc num :

P01225

ncbi mol weight :

14,700 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); FSH Signaling Pathway (672455); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Glycoprotein Hormones Pathway (160989); GnRH Signaling Pathway (83091); GnRH Signaling Pathway (502)

ncbi summary :

The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

uniprot summary :

FSHB: Stimulates development of follicle and spermatogenesis in the reproductive organs. Defects in FSHB are a cause of isolated follicle- stimulating hormone deficiency (IFSHD). Selective follicle-stimulating hormone deficiency is an uncommon cause of infertility, producing amenorrhea and hypogonadism in women and oligo or azoospermia with normal testosterone levels in normally virilised men. Belongs to the glycoprotein hormones subunit beta family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11p13. Cellular Component: cytoplasm; extracellular region. Molecular Function: follicle-stimulating hormone activity; protein binding; hormone activity. Biological Process: progesterone biosynthetic process; follicle-stimulating hormone signaling pathway; peptide hormone processing; female gamete generation; Sertoli cell proliferation; female pregnancy; signal transduction; regulation of osteoclast differentiation; cellular protein metabolic process; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of bone resorption; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell migration. Disease: Follicle-stimulating Hormone Deficiency, Isolated

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

400

size3 :

96-Strip-Wells

price3 :

530

size4 :

5x96-Strip-Wells

price4 :

2120

size5 :

10x96-Strip-Wells

price5 :

3820

more info or order :

MyBioSource product webpage