ELISA/assay

Myosin Binding Protein C, Cardiac (MYBPC3) ELISA Kit

MBS2021826

24-Strip-Wells

270 USD

ELISA Kit

Myosin Binding Protein C, Cardiac (MYBPC3) ELISA Kit

[Myosin Binding Protein C, Cardiac (MYBPC3)]

[MYBPC-3; FHC; CMH4; MYBP-C; C-protein, cardiac muscle isoform]

[Myosin binding protein C, cardiac; Myosin-binding protein C, cardiac-type; myosin-binding protein C, cardiac-type; myosin binding protein C, cardiac; C-protein, cardiac muscle isoform]

[MYBPC3]

[MYBPC3; MYBPC3; FHC; CMH4; CMD1MM; LVNC10; MYBP-C; Cardiac MyBP-C]

MYPC3_HUMAN

Human

1,273

This assay has high sensitivity and excellent specificity for detection of Myosin Binding Protein C, Cardiac (MYBPC3). No significant cross-reactivity or interference between Myosin Binding Protein C, Cardiac (MYBPC3) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids. Detection Range: 0.156-10ng/mL. Sensitivity: < 0.063ng/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Binding Protein C, Cardiac (MYBPC3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Binding Protein C, Cardiac (MYBPC3) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Cardiovascular biology

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Binding Protein C, Cardiac (MYBPC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Binding Protein C, Cardiac (MYBPC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Binding Protein C, Cardiac (MYBPC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Myosin Binding Protein C, Cardiac (MYBPC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

153217306

AAI51212.1

Q14896

140,703 Da

Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (106261); Striated Muscle Contraction Pathway (198903); Striated Muscle Contraction Pathway (106262)

MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

MYBPC3: Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Belongs to the immunoglobulin superfamily. MyBP family. Protein type: Myosin-binding. Chromosomal Location of Human Ortholog: 11p11.2. Cellular Component: sarcomere; striated muscle thick filament; cytosol; A band. Molecular Function: identical protein binding; ATPase activator activity; myosin binding; structural constituent of muscle; metal ion binding; titin binding; actin binding; myosin heavy chain binding. Biological Process: heart morphogenesis; regulation of heart rate; positive regulation of ATPase activity; regulation of muscle filament sliding; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; sarcomere organization; cell adhesion; myosin filament assembly; muscle filament sliding; cardiac muscle contraction. Disease: Left Ventricular Noncompaction 10; Cardiomyopathy, Familial Hypertrophic, 4

24-Strip-Wells

270 USD

48-Strip-Wells

495

96-Strip-Wells

665

5x96-Strip-Wells

2715

10x96-Strip-Wells

4895