ELISA/assay

Ghrelin (GHRL) ELISA Kit

MBS2021796

24-Strip-Wells

255 USD

ELISA Kit

Ghrelin (GHRL) ELISA Kit

[Ghrelin (GHRL)]

[MTLRP; Growth Hormone-Releasing Peptide]

[ghrelin; Ghrelin; appetite-regulating hormone; ghrelin/obestatin prepropeptide; Ghrelin]

[GHRL]

[GHRL; MTLRP]

E7CCH4_HUMAN

Human

117

This assay has high sensitivity and excellent specificity for detection of ghrelin. No significant cross-reactivity or interference between ghrelin and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Gallus Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 123.5-10,000pg/mL. Sensitivity: 48.4pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ghrelin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ghrelin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Signal transduction; Metabolic pathway; Endocrinology; Gastroenterology; Hormone metabolism

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ghrelin in gallus serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to ghrelin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled ghrelin and unlabeled ghrelin (Standards or samples) with the pre-coated antibody specific to ghrelin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of ghrelin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of ghrelin in the sample.

304435649

ADM33790.1

12,992 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); G Alpha (q) Signalling Events Pathway (1269578); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1269592); Metabolism Of Proteins Pathway (1268677); Peptide Hormone Metabolism Pathway (1268746); Peptide Ligand-binding Receptors Pathway (1269546); Signal Transduction Pathway (1269379); Signaling By GPCR Pathway (1269543)

This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]

ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach. Protein type: Secreted, signal peptide; Cell cycle regulation; Apoptosis; Secreted; Cell development/differentiation. Chromosomal Location of Human Ortholog: 3p26-p25. Cellular Component: extracellular space; axon; endoplasmic reticulum lumen; extracellular region. Molecular Function: growth hormone-releasing hormone activity; G-protein-coupled receptor binding; ghrelin receptor binding; protein tyrosine kinase activator activity. Biological Process: cortisol secretion; negative regulation of circadian sleep/wake cycle, REM sleep; positive regulation of cortisol secretion; hormone-mediated signaling; activation of MAPK activity; response to hormone stimulus; negative regulation of interleukin-6 biosynthetic process; positive regulation of multicellular organism growth; decidualization; negative regulation of insulin secretion; positive regulation of adrenocorticotropic hormone secretion; growth hormone secretion; elevation of cytosolic calcium ion concentration; negative regulation of locomotion; positive regulation of appetite; dendrite development; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of circadian sleep/wake cycle, non-REM sleep; regulation of response to food; gastric acid secretion; glucose metabolic process; positive regulation of insulin secretion; adult feeding behavior; negative regulation of interleukin-1 beta production; regulation of cell proliferation; positive regulation of growth hormone secretion; positive regulation of synaptogenesis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; cellular protein metabolic process; response to estrogen stimulus; actin polymerization and/or depolymerization; cartilage development; negative regulation of inflammatory response; negative regulation of endothelial cell proliferation; regulation of excitatory postsynaptic membrane potential; negative regulation of apoptosis. Disease: Obesity

24-Strip-Wells

255 USD

48-Strip-Wells

455

96-Strip-Wells

610

5x96-Strip-Wells

2470

10x96-Strip-Wells

4445