ELISA/assay

Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit

MBS2021763

48-Strip-Wells

540 USD

ELISA Kit

Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit

Transforming Growth Factor Beta 3 (TGFb3)

TGF-B3; LAP; Latency-associated peptide

transforming growth factor beta 3, partial; Transforming growth factor beta-3; transforming growth factor beta-3; transforming growth factor, beta 3

TGFb3

TGFB3; TGFB3; ARVD; RNHF; ARVD1; TGF-beta3; TGF-beta-3; LAP

TGFB3_HUMAN

Human

309

This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 3 (TGFb3). No significant cross-reactivity or interference between Transforming Growth Factor Beta 3 (TGFb3) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 7.81-500pg/m. Sensitivity: Typically less than 2.9pg/m.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 3 (TGFb3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 3 (TGFb3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 3 (TGFb3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 3 (TGFb3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta 3 (TGFb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Transforming Growth Factor Beta 3 (TGFb3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

37683095

AAQ98618.1

P10600

35,708 Da

ALK1 Signaling Events Pathway (137968); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chronic Myeloid Leukemia Pathway (83116); Chronic Myeloid Leukemia Pathway (528); Colorectal Cancer Pathway (83106)

This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and is involved in embryogenesis and cell differentiation. Defects in this gene are a cause of familial arrhythmogenic right ventricular dysplasia 1. [provided by RefSeq, Mar 2009]

TGFB3: Involved in embryogenesis and cell differentiation. Homodimer; disulfide-linked. Interacts with ASPN. Belongs to the TGF-beta family. Protein type: Secreted, signal peptide; Ligand, receptor tyrosine kinase; Motility/polarity/chemotaxis; Secreted; Cell development/differentiation. Chromosomal Location of Human Ortholog: 14q24. Cellular Component: extracellular matrix; extracellular space; cell surface; cell soma; T-tubule; extracellular region; plasma membrane; nucleus. Molecular Function: identical protein binding; protein binding; growth factor activity; transforming growth factor beta binding; protein heterodimerization activity; punt binding; cytokine activity. Biological Process: extracellular matrix organization and biogenesis; positive regulation of apoptosis; activation of MAPK activity; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; palate development; SMAD protein nuclear translocation; female pregnancy; regulation of apoptosis; odontogenesis; negative regulation of cell proliferation; platelet degranulation; mammary gland development; transforming growth factor beta receptor signaling pathway; salivary gland morphogenesis; embryonic neurocranium morphogenesis; negative regulation of neuron apoptosis; cell growth; inner ear development; aging; uterine wall breakdown; positive regulation of filopodium formation; platelet activation; intercellular junction assembly and maintenance; in utero embryonic development; positive regulation of bone mineralization; regulation of cell proliferation; gut development; positive regulation of protein secretion; negative regulation of DNA replication; response to estrogen stimulus; positive regulation of cell division; regulation of MAPKKK cascade; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; response to progesterone stimulus; negative regulation of transforming growth factor beta receptor signaling pathway; alveolus development; positive regulation of DNA replication. Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 1; Rienhoff Syndrome

48-Strip-Wells

540 USD

96-Strip-Wells

730

5x96-Strip-Wells

2830

10x96-Strip-Wells

5080