ELISA/assay

Thy1 Cell Surface Antigen (Thy1) ELISA Kit

MBS2021759

48-Strip-Wells

550 USD

ELISA Kit

Thy1 Cell Surface Antigen (Thy1) ELISA Kit

Thy1 Cell Surface Antigen (Thy1)

CD90; CDw90; THY mocyte differentiation antigen 1

Thy-1 cell surface antigen, isoform CRA_a; Thy-1 membrane glycoprotein; thy-1 membrane glycoprotein; Thy-1 cell surface antigen; CDw90; Thy-1 antigen; CD_antigen: CD90

Thy1

THY1; THY1; CD90; CDw90

THY1_HUMAN

Mouse

161

This assay has high sensitivity and excellent specificity for detection of Thy1. No significant cross-reactivity or interference between Thy1 and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Mouse serum, plasma, urine, tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 12.5-800pg/mL. The standard curve concentrations used for the ELISA's were 800pg/mL, 400pg/mL, 200pg/mL, 100pg/mL, 50pg/mL, 25pg/mL, 12.5pg/mL. Sensitivity: The minimum detectable dose of Thy1 is typically less than 5.7pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Thy1 in mouse serum, plasma, urine, tissue homogenates, cell lysates and other biological fluids. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thy1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thy1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thy1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thy1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thy1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thy1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119587894

EAW67490.1

P04216

17,935 Da

Cardiac Progenitor Differentiation Pathway (712094); IL4-mediated Signaling Events Pathway (137933); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); Amb2 Integrin Signaling Pathway (137945)

This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 11q23.3. Cellular Component: anchored to external side of plasma membrane; focal adhesion; growth cone; endoplasmic reticulum; integral to plasma membrane; apical plasma membrane; dendrite; plasma membrane; cytosol; external side of plasma membrane; lipid raft. Molecular Function: integrin binding; protein binding; protein kinase binding. Biological Process: focal adhesion formation; cell-cell adhesion; retinal cone cell development; negative regulation of protein kinase activity; negative regulation of T cell receptor signaling pathway; cytoskeleton organization and biogenesis; negative regulation of axonogenesis; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of T cell activation; angiogenesis; T cell receptor signaling pathway; negative regulation of cell migration; positive regulation of GTPase activity

48-Strip-Wells

550 USD

96-Strip-Wells

750

5x96-Strip-Wells

2910

10x96-Strip-Wells

5220