ELISA/assay

Granulocyte Chemotactic Protein 2 (GCP2) ELISA Kit

MBS2021752

48-Strip-Wells

550 USD

ELISA Kit

Granulocyte Chemotactic Protein 2 (GCP2) ELISA Kit

Granulocyte Chemotactic Protein 2 (GCP2)

CXCL6; SCYB6; GCP2; CKA-3; Chemokine(C-X-C-Motif)ligand 6; Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 6; Chemokine alpha 3; Small-inducible cytokine B6

granulocyte chemotactic protein-2; C-X-C motif chemokine 6; C-X-C motif chemokine 6; chemokine (C-X-C motif) ligand 6; Chemokine alpha 3; CKA-3

GCP2

CXCL6; CXCL6; GCP2; CKA-3; GCP-2; SCYB6; GCP2; SCYB6; CKA-3; GCP-2

CXCL6_HUMAN

Mouse

114

This assay has high sensitivity and excellent specificity for detection of Granulocyte Chemotactic Protein 2 (GCP2). No significant cross-reactivity or interference between Granulocyte Chemotactic Protein 2 (GCP2) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 6.25-400pg/mL. Sensitivity: Typically less than 2.78pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Granulocyte Chemotactic Protein 2 (GCP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Granulocyte Chemotactic Protein 2 (GCP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granulocyte Chemotactic Protein 2 (GCP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Granulocyte Chemotactic Protein 2 (GCP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granulocyte Chemotactic Protein 2 (GCP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Granulocyte Chemotactic Protein 2 (GCP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

1916230

AAC51338.1

P80162

11,897 Da

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548)

CXCL6: Chemotactic for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular space; extracellular region. Molecular Function: heparin binding; chemokine activity; CXCR chemokine receptor binding. Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; defense response to bacterium; neutrophil mediated immunity; positive regulation of leukocyte chemotaxis; immune response; response to lipopolysaccharide; inflammatory response; signal transduction; chemotaxis; regulation of cell proliferation

48-Strip-Wells

550 USD

96-Strip-Wells

750

5x96-Strip-Wells

2910

10x96-Strip-Wells

5220