ELISA/assay

Thymidine Kinase 1, Soluble (TK1) ELISA Kit

MBS2021703

48-Strip-Wells

565 USD

ELISA Kit

Thymidine Kinase 1, Soluble (TK1) ELISA Kit

Thymidine Kinase 1, Soluble (TK1)

TK2; Thymidine kinase, cytosolic

Thymidine kinase 1, soluble; Thymidine kinase, cytosolic; thymidine kinase, cytosolic; thymidine kinase 1, soluble

TK1

TK1; TK1; TK2

KITH_HUMAN

Human

234

This assay has high sensitivity and excellent specificity for detection of Thymidine Kinase 1, Soluble (TK1). No significant cross-reactivity or interference between Thymidine Kinase 1, Soluble (TK1) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: Typically less than 5.9pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymidine Kinase 1, Soluble (TK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymidine Kinase 1, Soluble (TK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymidine Kinase 1, Soluble (TK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymidine Kinase 1, Soluble (TK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymidine Kinase 1, Soluble (TK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Thymidine Kinase 1, Soluble (TK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

52632392

AAH06484.1

P04183

25,469 Da

Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); E2F Transcription Factor Network Pathway (137934); Fluoropyrimidine Activity Pathway (198778); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Pyrimidine Metabolism Pathway (106281); Pyrimidine Metabolism Pathway (82946); Pyrimidine Metabolism Pathway (309)

TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells. Protein type: EC 2.7.1.21; Xenobiotic Metabolism - drug metabolism - other enzymes; Kinase, other; Nucleotide Metabolism - pyrimidine. Chromosomal Location of Human Ortholog: 17q23.2-q25.3. Cellular Component: cytosol. Molecular Function: identical protein binding; nucleoside kinase activity; zinc ion binding; thymidine kinase activity; ATP binding. Biological Process: response to nutrient levels; skeletal muscle cell proliferation; nucleobase, nucleoside and nucleotide metabolic process; response to toxin; liver development; deoxyribonucleoside monophosphate biosynthetic process; protein homotetramerization; fetal process involved in parturition; response to cortisol stimulus; pyrimidine base metabolic process; gut development; response to copper ion; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; pyrimidine nucleoside salvage; thymidine metabolic process; phosphorylation

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340