Growth Differentiation Factor 11 (GDF11) ELISA Kit | MyBioSource MBS2021645 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Growth Differentiation Factor 11 (GDF11) ELISA Kit

catalog :

MBS2021645

quantity :

24-Strip-Wells

price :

270 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2021645

products type :

ELISA Kit

products full name :

Growth Differentiation Factor 11 (GDF11) ELISA Kit

products short name :

[Growth Differentiation Factor 11 (GDF11)]

products name syn :

[BMP11; Bone morphogenetic protein 11]

other names :

[growth differentiation factor 11, isoform CRA_a; Growth/differentiation factor 11; growth/differentiation factor 11; growth differentiation factor 11; Bone morphogenetic protein 11; BMP-11]

products gene name :

[GDF11]

other gene names :

[GDF11; GDF11; BMP11; BMP-11; BMP11; GDF-11; BMP-11]

uniprot entry name :

GDF11_HUMAN

reactivity :

Mouse

sequence length :

407

specificity :

This assay has high sensitivity and excellent specificity for detection of GDF11. No significant cross-reactivity or interference between GDF11 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 12.5-800pg/mL. Sensitivity: 4.8pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GDF11 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GDF11 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Cytokine; Neuroscience; Ophthalmology & Otorhinolaryngology; Developmental science; Bone metabolism

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GDF11 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GDF11. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GDF11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GDF11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GDF11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

119617237

ncbi acc num :

EAW96831.1

uniprot acc num :

O95390

ncbi mol weight :

45,091 Da

ncbi summary :

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development. [provided by RefSeq, Jul 2008]

uniprot summary :

GDF11: Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern. Belongs to the TGF-beta family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 12q13.2. Cellular Component: extracellular space. Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding. Biological Process: regulation of apoptosis; negative regulation of cell proliferation; nervous system development; negative regulation of cell differentiation; camera-type eye morphogenesis; spinal cord anterior/posterior patterning; ureteric bud development; regulation of MAPKKK cascade; cell maturation; pancreas development; mesoderm development; palate development; skeletal development; cell development; metanephros development; growth

size1 :

24-Strip-Wells

price1 :

270 USD

size2 :

48-Strip-Wells

price2 :

505

size3 :

96-Strip-Wells

price3 :

685

size4 :

5x96-Strip-Wells

price4 :

2770

size5 :

10x96-Strip-Wells

price5 :

5025

more info or order :

MyBioSource product webpage