ELISA/assay

Defensin Beta 126 (DEFb126) ELISA Kit

MBS2021586

96 Strip Wells

845 USD

ELISA Kit

Defensin Beta 126 (DEFb126) ELISA Kit

Defensin Beta 126 (DEFb126)

ESP13.2; C20orf8; DEFB-26; Epididymal secretory protein 13.2

defensin beta 126, partial; Beta-defensin 126; beta-defensin 126; defensin, beta 126; Beta-defensin 26; DEFB-26; Defensin, beta 126; Epididymal secretory protein 13.2; ESP13.2

DEFb126

DEFB126; DEFB126; DEFB26; hBD-26; C20orf8; DEFB-26; bA530N10.1; C20orf8; DEFB26; DEFB-26; ESP13.2

DB126_HUMAN

Human

111

This assay has high sensitivity and excellent specificity for detection of Defensin Beta 126 (DEFb126). No significant cross-reactivity or interference between Defensin Beta 126 (DEFb126) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Sample Types: Serum, plasma and other biological fluids. Detection Range: 7.81-500pg/mL. Sensitivity: The minimum detectable dose of this kit is typically less than 3.4pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Beta 126 (DEFb126) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Beta 126 (DEFb126) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 126 (DEFb126). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 126 (DEFb126). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 126 (DEFb126), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Defensin Beta 126 (DEFb126) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

22252927

AAM93919.1

Q9BYW3

12,174 Da

Beta Defensins Pathway 530759!!Defensins Pathway 530757!!Immune System Pathway 106386!!Innate Immune System Pathway 106387

Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The antimicrobial protein encoded by this gene is secreted and is a member of the beta defensin protein family. Beta defensin genes are found in several clusters throughout the genome, with this gene mapping to a cluster at 20p13. The encoded protein is highly similar to an epididymal-specific secretory protein (ESP13.2) from cynomolgus monkey. Mutation of this gene is associated with impaired sperm function. [provided by RefSeq, Nov 2014]

96 Strip Wells

845 USD