Prostate Stem Cell Antigen (PSCA) ELISA Kit | MyBioSource MBS2021570 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Prostate Stem Cell Antigen (PSCA) ELISA Kit

catalog :

MBS2021570

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2021570

products type :

ELISA Kit

products full name :

Prostate Stem Cell Antigen (PSCA) ELISA Kit

products short name :

Prostate Stem Cell Antigen (PSCA)

other names :

prostate stem cell antigen preproprotein; Prostate stem cell antigen; prostate stem cell antigen; prostate stem cell antigen

products gene name :

PSCA

other gene names :

PSCA; PSCA; PRO232

uniprot entry name :

PSCA_HUMAN

reactivity :

Human

sequence length :

123

specificity :

This assay has high sensitivity and excellent specificity for detection of Prostate Stem Cell Antigen (PSCA). No significant cross-reactivity or interference between Prostate Stem Cell Antigen (PSCA) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.063ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostate Stem Cell Antigen (PSCA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostate Stem Cell Antigen (PSCA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostate Stem Cell Antigen (PSCA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostate Stem Cell Antigen (PSCA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostate Stem Cell Antigen (PSCA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Prostate Stem Cell Antigen (PSCA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

289547757

ncbi acc num :

NP_005663.2

ncbi gb acc num :

NM_005672.4

uniprot acc num :

O43653

ncbi mol weight :

12,912 Da

ncbi summary :

This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder, placenta, colon, kidney, and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene includes a polymorphism that results in an upstream start codon in some individuals; this polymorphism is thought to be associated with a risk for certain gastric and bladder cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

uniprot summary :

PSCA: May be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro. Protein type: Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 8q24.2. Cellular Component: plasma membrane

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage