ELISA/assay

Terminal Complement Complex C5b-9 (C5b-9) ELISA Kit

MBS2021557

24-Strip-Wells

270 USD

ELISA Kit

Terminal Complement Complex C5b-9 (C5b-9) ELISA Kit

[Terminal Complement Complex C5b-9 (C5b-9)]

[MAC; Membrane Attack Complex]

[C5b-9]

Human

This assay has high sensitivity and excellent specificity for detection of C5a. No significant cross-reactivity or interference between C5a and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.57ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C5a were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C5a were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Signal transduction; Metabolic pathway; Apoptosis; Infection immunity

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of C5a in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to C5a. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to C5a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C5a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C5a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

24-Strip-Wells

270 USD

48-Strip-Wells

495

96-Strip-Wells

665

5x96-Strip-Wells

2715

10x96-Strip-Wells

4895