Ki-67 Protein (Ki67P) ELISA Kit | MyBioSource MBS2021528 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Ki-67 Protein (Ki67P) ELISA Kit

catalog :

MBS2021528

quantity :

48-Strip-Wells

price :

605 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2021528

products type :

ELISA Kit

products full name :

Ki-67 Protein (Ki67P) ELISA Kit

products short name :

Ki-67 Protein (Ki67P)

products name syn :

MKI67; KIA; Ki-67; Antigen Identified By Monoclonal Antibody Ki-67

other names :

Ki-67; Ki-67 protein; Ki-67 protein

products gene name :

Ki67P

other gene names :

Mki67

uniprot entry name :

Q61769_MOUSE

reactivity :

Rat

sequence length :

2,938

specificity :

This assay has high sensitivity and excellent specificity for detection of Ki-67 Protein (Ki67P). No significant cross-reactivity or interference between Ki-67 Protein (Ki67P) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Typically less than 0.115ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ki-67 Protein (Ki67P) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ki-67 Protein (Ki67P) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ki-67 Protein (Ki67P). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ki-67 Protein (Ki67P). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ki-67 Protein (Ki67P), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Ki-67 Protein (Ki67P) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

1177528

ncbi acc num :

CAA58026.1

uniprot acc num :

Q61769

ncbi mol weight :

324,429 Da

uniprot summary :

KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers. Protein type: Nucleolus; Cell cycle regulation. Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region. Molecular Function: protein C-terminus binding. Biological Process: meiosis; cell proliferation

size1 :

48-Strip-Wells

price1 :

605 USD

size2 :

96-Strip-Wells

price2 :

820

size3 :

5x96-Strip-Wells

price3 :

3220

size4 :

10x96-Strip-Wells

price4 :

5795

more info or order :

MyBioSource product webpage