ELISA/assay

Ornithine Decarboxylase (ODC) ELISA Kit

MBS2021522

48-Strip-Wells

540 USD

ELISA Kit

Ornithine Decarboxylase (ODC) ELISA Kit

Ornithine Decarboxylase (ODC)

ODC1

ornithine decarboxylase; Ornithine decarboxylase; ornithine decarboxylase; ornithine decarboxylase 1

ODC

Odc1; Odc1; Odc; Odc; ODC

DCOR_RAT

Human

461

This assay has high sensitivity and excellent specificity for detection of Ornithine Decarboxylase (ODC). No significant cross-reactivity or interference between Ornithine Decarboxylase (ODC) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Typically less than 0.127ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ornithine Decarboxylase (ODC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ornithine Decarboxylase (ODC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ornithine Decarboxylase (ODC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ornithine Decarboxylase (ODC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ornithine Decarboxylase (ODC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Ornithine Decarboxylase (ODC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

6981302

NP_036747.1

NM_012615.3

P09057

51,047 Da

Arginine And Proline Metabolism Pathway (83349); Arginine And Proline Metabolism Pathway (323); Glutathione Metabolism Pathway (83365); Glutathione Metabolism Pathway (343); Metabolic Pathways (132933); Polyamine Biosynthesis, Arginine = Ornithine = Putrescine Pathway (434717); Polyamine Biosynthesis, Arginine = Ornithine = Putrescine Pathway (468321); Urea Cycle And Metabolism Of Amino Groups Pathway (198443)

catalyzes the conversion of L-ornithine to putrescine [RGD, Feb 2006]

ODC: the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. [provided by RefSeq, Jul 2008]. Protein type: Other Amino Acids Metabolism - glutathione; Amino Acid Metabolism - arginine and proline; Cell cycle regulation; Lyase; EC 4.1.1.17. Cellular Component: perinuclear region of cytoplasm; cytoplasm; cytosol. Molecular Function: protein homodimerization activity; ornithine decarboxylase activity. Biological Process: response to virus; putrescine biosynthetic process; positive regulation of cell proliferation; regulation of protein catabolic process; kidney development; putrescine biosynthetic process from ornithine; polyamine metabolic process

48-Strip-Wells

540 USD

96-Strip-Wells

730

5x96-Strip-Wells

2830

10x96-Strip-Wells

5080