ELISA/assay

ELISA Kit for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)

MBS2021425

48-Strip-Wells

640 USD

ELISA Kit

ELISA Kit for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)

Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)

Wingless-type MMTV integration site family, member 5A; Protein Wnt-5a; protein Wnt-5a; WNT-5A protein; wingless-type MMTV integration site family, member 5A

WNT5A

WNT5A; WNT5A; hWNT5A

WNT5A_HUMAN

Mouse

380

This assay has high sensitivity and excellent specificity for detection of WNT5A. No significant cross-reactivity or interference between WNT5A and analogues was observed.

For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.

Samples: Mouse tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Detection Range: 0.78 -50ng/mL. Sensitivity: The minimum detectable dose of WNT5A is typically less than 0.33ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT5A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT5A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of WNT5A in mouse tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT5A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of WNT5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

40352783

AAH64694.1

P41221

40,887 Da

Asymmetric Localization Of PCP Proteins Pathway (1127529); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Ca2+ Pathway (1127532); Class B/2 (Secretin Family Receptors) Pathway (106378); DNA Damage Response (only ATM Dependent) Pathway (198827); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); GPCR Ligand Binding Pathway (161020); HTLV-I Infection Pathway (373901)

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

WNT5A: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. Interacts with PORCN. Interacts with WLS. Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3p21-p14. Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; Golgi lumen; endoplasmic reticulum lumen; plasma membrane; extracellular region. Molecular Function: protein domain specific binding; receptor tyrosine kinase-like orphan receptor binding; frizzled binding; cytokine activity; receptor agonist activity; transcription factor activity; frizzled-2 binding. Biological Process: activation of MAPK activity; positive regulation of transcription, DNA-dependent; embryonic skeletal development; negative chemotaxis; negative regulation of synaptogenesis; Wnt receptor signaling pathway through beta-catenin; positive regulation of interleukin-1 beta secretion; uterus development; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; negative regulation of BMP signaling pathway; neuron differentiation; positive regulation of fibroblast proliferation; determination of anterior/posterior axis, embryo; positive regulation of mesenchymal cell proliferation; positive regulation of macrophage activation; somitogenesis; cell fate commitment; urinary bladder development; olfactory bulb interneuron development; activation of protein kinase B; positive regulation of interleukin-6 production; vagina development; negative regulation of fat cell differentiation; keratinocyte differentiation; genitalia development; Wnt receptor signaling pathway, calcium modulating pathway; positive regulation of angiogenesis; positive regulation of protein catabolic process; midgut development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; lens development in camera-type eye; axon guidance; wound healing; hypophysis morphogenesis; negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of cytokine secretion during immune response; palate development; negative regulation of axon extension involved in axon guidance; activation of JNK activity; response to organic substance; establishment of planar polarity; heart looping; negative regulation of epithelial cell proliferation; cervix development; Wnt receptor signaling pathway; positive regulation of meiosis; male gonad development; positive regulation of peptidyl-serine phosphorylation; positive regulation of ossification; positive regulation of cGMP metabolic process; positive regulation of interferon-gamma production; convergent extension involved in organogenesis; positive regulation of chemokine biosynthetic process; ameboidal cell migration; cartilage development; neural tube closure; epithelial to mesenchymal transition; hindgut morphogenesis; lung development; positive regulation of inflammatory response. Disease: Robinow Syndrome, Autosomal Dominant

48-Strip-Wells

640 USD

96-Strip-Wells

870

5x96-Strip-Wells

3355

10x96-Strip-Wells

6035