ELISA/assay

Angiopoietin Like Protein 2 (ANGPTL2) ELISA Kit

MBS2021399

24-Strip-Wells

260 USD

ELISA Kit

Angiopoietin Like Protein 2 (ANGPTL2) ELISA Kit

[Angiopoietin Like Protein 2 (ANGPTL2)]

[HARP; ARP2; Angiopoietin-Related Protein 2]

[angiopoietin-related protein 2; Angiopoietin-related protein 2; Angiopoietin-like protein 2]

[ANGPTL2]

[ANGPTL2; ARP2]

ANGL2_HUMAN

Human

191

This assay has high sensitivity and excellent specificity for detection of Angiopoietin Like Protein 2 (ANGPTL2). No significant cross-reactivity or interference between Angiopoietin Like Protein 2 (ANGPTL2) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 0.312-20ng/mL. Sensitivity: < 0.119ng/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin Like Protein 2 (ANGPTL2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin Like Protein 2 (ANGPTL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Tumor immunity; Infection immunity; Cardiovascular biology

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 2 (ANGPTL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin Like Protein 2 (ANGPTL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 2 (ANGPTL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Angiopoietin Like Protein 2 (ANGPTL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

6912236

NP_036230.1

NM_012098.2

Q9UKU9

22,717 Da

Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action. [provided by RefSeq, Jul 2008]

ANGPTL2: Induces sprouting in endothelial cells through an autocrine and paracrine action. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: extracellular space. Molecular Function: receptor binding. Biological Process: multicellular organismal development

24-Strip-Wells

260 USD

48-Strip-Wells

475

96-Strip-Wells

640

5x96-Strip-Wells

2590

10x96-Strip-Wells

4670