ELISA/assay

S100 Calcium Binding Protein A10 (S100A10) ELISA Kit

MBS2021386

48-Strip-Wells

470 USD

ELISA Kit

S100 Calcium Binding Protein A10 (S100A10) ELISA Kit

S100 Calcium Binding Protein A10 (S100A10)

ANX2L; ANX2LG; CAL1L; CLP11; GP11; P11; P10; Calpactin-1 light chain; Cellular ligand of annexin II; Annexin II Ligand,Calpactin I,Light Polypeptide

S100 calcium binding protein A10; Protein S100-A10; protein S100-A10; S100 calcium binding protein A10; Calpactin I light chain; Calpactin-1 light chain; Cellular ligand of annexin II; S100 calcium-binding protein A10; p10 protein; p11

S100A10

S100A10; S100A10; 42C; P11; p10; GP11; ANX2L; CAL1L; CLP11; Ca[1]; ANX2LG; ANX2LG; CAL1L; CLP11

S10AA_HUMAN

Human

97

This assay has high sensitivity and excellent specificity for detection of S100A10. No significant cross-reactivity or interference between S100A10 and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Human serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of S100A10 is typically less than 0.063ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100A10 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100A10 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of S100A10 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100A10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100A10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100A10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100A10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

76779210

AAI05787.1

P60903

11,203 Da

Dissolution Of Fibrin Clot Pathway (106061); Hemostasis Pathway (106028); Orphan Transporters Pathway (1127546); Prostaglandin Synthesis And Regulation Pathway (198912); Transmembrane Transport Of Small Molecules Pathway (106572)

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis. [provided by RefSeq, Jul 2008]

S100A10: Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine- specific kinase. Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with SCN10A. Belongs to the S-100 family. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extrinsic to plasma membrane; lipid raft. Molecular Function: protein binding; protein homodimerization activity; calcium ion binding; lipid binding. Biological Process: positive regulation of focal adhesion formation; positive regulation of stress fiber formation; protein heterotetramerization; positive regulation of binding; lipid raft formation; membrane budding

48-Strip-Wells

470 USD

96-Strip-Wells

630

5x96-Strip-Wells

2410

10x96-Strip-Wells

4320