ELISA/assay

Follistatin Like Protein 3 (FSTL3) ELISA Kit

MBS2021344

48-Strip-Wells

575 USD

ELISA Kit

Follistatin Like Protein 3 (FSTL3) ELISA Kit

Follistatin Like Protein 3 (FSTL3)

FLRG; FSRP; Follistatin-Related Protein,Secreted Glycoprotein; Follistatin-related gene protein

follistatin-related protein 3; Follistatin-related protein 3; follistatin-related protein 3; follistatin-like 3 (secreted glycoprotein); Follistatin-like protein 3; Follistatin-related gene protein

FSTL3

FSTL3; FSTL3; FLRG; FSRP; FLRG

FSTL3_HUMAN

Mouse

237

This assay has high sensitivity and excellent specificity for detection of Follistatin Like Protein 3 (FSTL3). No significant cross-reactivity or interference between Follistatin Like Protein 3 (FSTL3) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: Typically less than 5.8pg/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follistatin Like Protein 3 (FSTL3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follistatin Like Protein 3 (FSTL3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Follistatin Like Protein 3 (FSTL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Follistatin Like Protein 3 (FSTL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Follistatin Like Protein 3 (FSTL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Follistatin Like Protein 3 (FSTL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

5031701

NP_005851.1

NM_005860.2

O95633

24,960 Da

Antagonism Of Activin By Follistatin Pathway (730350); Signal Transduction Pathway (477114); Signaling By Activin Pathway (730349)

Follistatin-like 3 is a secreted glycoprotein of the follistatin-module-protein family. It may have a role in leukemogenesis. [provided by RefSeq, Jul 2008]

FSTL3: Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10. A chromosomal aberration involving FSTL3 is found in a case of B-cell chronic lymphocytic leukemia. Translocation t(11;19)(q13;p13) with CCDN1. 2 isoforms of the human protein are produced by alternative initiation. Protein type: Secreted; Oncoprotein; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19p13. Cellular Component: nucleoplasm; Golgi apparatus; extracellular space; extracellular region; nucleus; secretory granule. Molecular Function: protein binding; fibronectin binding; activin binding. Biological Process: negative regulation of osteoclast differentiation; ossification; transcription, DNA-dependent; adrenal gland development; male gonad development; negative regulation of BMP signaling pathway; regulation of transcription from RNA polymerase II promoter; negative regulation of activin receptor signaling pathway; positive regulation of cell-cell adhesion; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; spermatogenesis; kidney development; lung development

48-Strip-Wells

575 USD

96-Strip-Wells

785

5x96-Strip-Wells

3055

10x96-Strip-Wells

5495