Chordin (CHRD) ELISA Kit | MyBioSource MBS2021329 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Chordin (CHRD) ELISA Kit

catalog :

MBS2021329

quantity :

48-Strip-Wells

price :

605 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2021329

products type :

ELISA Kit

products full name :

Chordin (CHRD) ELISA Kit

products short name :

Chordin (CHRD)

other names :

chordin; Chordin; chordin; chordin

products gene name :

CHRD

other gene names :

CHRD; CHRD

uniprot entry name :

CHRD_HUMAN

reactivity :

Rat

sequence length :

915

specificity :

This assay has high sensitivity and excellent specificity for detection of Chordin (CHRD). No significant cross-reactivity or interference between Chordin (CHRD) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.056ng/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chordin (CHRD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chordin (CHRD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chordin (CHRD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chordin (CHRD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chordin (CHRD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Chordin (CHRD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

11494125

ncbi acc num :

AAG35767.1

uniprot acc num :

Q9H2X0

ncbi mol weight :

97,731 Da

ncbi pathways :

BMP Receptor Signaling Pathway (137948); TGF-beta Signaling Pathway (83064); TGF-beta Signaling Pathway (475)

ncbi summary :

This gene encodes a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. The encoded protein may also have roles in organogenesis and during adulthood. It has been suggested that this gene could be a candidate gene for Cornelia de Lange syndrome. Reduced expression of this gene results in enhanced bone regeneration. Alternative splicing results in multiple transcript variants. Other alternative splice variants have been described but their full length sequence has not been determined. [provided by RefSeq, Jan 2015]

uniprot summary :

CHRD: Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes. Belongs to the chordin family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Cell adhesion; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3q27. Cellular Component: extracellular space. Molecular Function: heparin binding; syndecan binding; protein binding; cytokine binding. Biological Process: BMP signaling pathway in spinal cord dorsal-ventral patterning; osteoblast differentiation; floor plate development; positive regulation of cell adhesion; mesoderm formation; positive regulation of mesenchymal cell proliferation; forebrain development; negative regulation of osteoblast differentiation; gastrulation with mouth forming second; skeletal development; negative regulation of cell migration; negative regulation of BMP signaling pathway

size1 :

48-Strip-Wells

price1 :

605 USD

size2 :

96-Strip-Wells

price2 :

820

size3 :

5x96-Strip-Wells

price3 :

3220

size4 :

10x96-Strip-Wells

price4 :

5795

more info or order :

MyBioSource product webpage