Glucagon Like Peptide 1 Receptor (GLP1R) ELISA Kit | MyBioSource MBS2021324 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Glucagon Like Peptide 1 Receptor (GLP1R) ELISA Kit

catalog :

MBS2021324

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2021324

products type :

ELISA Kit

products full name :

Glucagon Like Peptide 1 Receptor (GLP1R) ELISA Kit

products short name :

Glucagon Like Peptide 1 Receptor (GLP1R)

other names :

glucagon-like peptide 1 receptor; Glucagon-like peptide 1 receptor; glucagon-like peptide 1 receptor; glucagon-like peptide 1 receptor

products gene name :

GLP1R

other gene names :

GLP1R; GLP1R; GLP-1 receptor; GLP-1-R; GLP-1R

uniprot entry name :

GLP1R_HUMAN

reactivity :

Human

sequence length :

463

specificity :

This assay has high sensitivity and excellent specificity for detection of GLP1R. No significant cross-reactivity or interference between GLP1R and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Human tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. The standard curve concentrations used for the ELISA's were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL. Sensitivity: 0.055ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GLP1R were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GLP1R were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GLP1R in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to GLP1R. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GLP1R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GLP1R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GLP1R in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

166795283

ncbi acc num :

NP_002053.3

ncbi gb acc num :

NM_002062.3

uniprot acc num :

P43220

ncbi mol weight :

53,026 Da

ncbi pathways :

Class B/2 (Secretin Family Receptors) Pathway (106378); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class B Secretin-like Pathway (198781); Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway (105925); Glucagon-type Ligand Receptors Pathway (106380); Insulin Secretion Pathway (777534)

uniprot summary :

GLP1R: This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family. Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 2; Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21. Cellular Component: integral to membrane; plasma membrane. Molecular Function: transmembrane receptor activity; glucagon receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; elevation of cytosolic calcium ion concentration; learning and/or memory; adenylate cyclase activation; energy reserve metabolic process; response to stress; regulation of insulin secretion; regulation of heart contraction

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage