ELISA/assay

Lysyl Oxidase Like Protein 2 (LOXL2) ELISA Kit

MBS2021230

48-Strip-Wells

575 USD

ELISA Kit

Lysyl Oxidase Like Protein 2 (LOXL2) ELISA Kit

Lysyl Oxidase Like Protein 2 (LOXL2)

LOR2; WS9-14; Lysyl oxidase homolog 2; Lysyl oxidase-related protein 2; Lysyl oxidase-related protein WS9-14

lysyl oxidase homolog 2; Lysyl oxidase homolog 2; lysyl oxidase homolog 2; lysyl oxidase-like 2; Lysyl oxidase-like protein 2; Lysyl oxidase-related protein 2; Lysyl oxidase-related protein WS9-14

LOXL2

LOXL2; LOXL2; LOR2; WS9-14

LOXL2_HUMAN

Mouse

774

This assay has high sensitivity and excellent specificity for detection of LOXL2. No significant cross-reactivity or interference between LOXL2 and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Mouse serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. The standard curve concentrations used for the ELISA's were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL. Sensitivity: Typically less than 0.067ng/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LOXL2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LOXL2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LOXL2 in mouse serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to LOXL2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to LOXL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LOXL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LOXL2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4505011

NP_002309.1

NM_002318.2

Q9Y4K0

86,725 Da

Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Formation Pathway (645288); Crosslinking Of Collagen Fibrils Pathway (730308); Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262)

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]

LOXL2: Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated Lys-4 of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E- cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation. Belongs to the lysyl oxidase family. Protein type: Secreted, signal peptide; Cell adhesion; Secreted; EC 1.4.3.13; Oxidoreductase. Chromosomal Location of Human Ortholog: 8p21.3. Cellular Component: nucleoplasm; extracellular space; membrane; basement membrane; chromosome; nucleus. Molecular Function: protein binding; copper ion binding; electron carrier activity; protein-lysine 6-oxidase activity; chromatin binding; transcription corepressor activity; methylated histone residue binding; scavenger receptor activity. Biological Process: receptor-mediated endocytosis; collagen fibril organization; transcription, DNA-dependent; protein modification process; protein amino acid deamination; positive regulation of chondrocyte differentiation; response to copper ion; endothelial cell proliferation; histone modification; response to hypoxia; epithelial to mesenchymal transition; endothelial cell migration; sprouting angiogenesis; cell adhesion; negative regulation of transcription, DNA-dependent; aging

48-Strip-Wells

575 USD

96-Strip-Wells

785

5x96-Strip-Wells

3055

10x96-Strip-Wells

5495