ELISA/assay

High Mobility Group Protein 1 (HMG1) ELISA Kit

MBS2021229

48-Strip-Wells

515 USD

ELISA Kit

High Mobility Group Protein 1 (HMG1) ELISA Kit

High Mobility Group Protein 1 (HMG1)

HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein

high mobility group protein B1; High mobility group protein B1; high mobility group protein B1; high mobility group box 1; High mobility group protein 1; HMG-1

HMG1

HMGB1; HMGB1; HMG1; HMG3; SBP-1; HMG1; HMG-1

HMGB1_HUMAN

Human

215

This assay has high sensitivity and excellent specificity for detection of HMG1. No significant cross-reactivity or interference between HMG1 and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Human serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 62.5-4,000pg/mL. The standard curve concentrations used for the ELISA's were 4,000pg/mL, 2,000pg/mL, 1,000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL. Sensitivity: 29.4pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HMG1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HMG1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HMG1 in human serum, plasma and other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to HMG1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HMG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HMG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HMG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4504425

NP_002119.1

NM_002128.4

P09429

24,894 Da

Activated TLR4 Signalling Pathway (106400); Activation Of DNA Fragmentation Factor Pathway (105683); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Apoptosis Pathway (105648); Apoptosis Induced DNA Fragmentation Pathway (105682); Apoptotic Execution Phase Pathway (105677); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (833822)

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family. Protein type: Nuclear receptor co-regulator; DNA repair, damage. Chromosomal Location of Human Ortholog: 13q12. Cellular Component: nucleoplasm; extracellular space; cell surface; transcriptional repressor complex; extracellular region; condensed chromosome; nucleus. Molecular Function: RAGE receptor binding; protein binding; double-stranded DNA binding; damaged DNA binding; cytokine activity; chromatin binding; DNA bending activity; transcription factor binding; transcription factor activity; single-stranded DNA binding; chemoattractant activity. Biological Process: negative regulation of transcriptional preinitiation complex assembly; V(D)J recombination; DNA topological change; apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; base-excision repair, DNA ligation; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; regulation of transcription from RNA polymerase II promoter; chromatin remodeling; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; positive chemotaxis; DNA ligation during DNA repair; dendritic cell chemotaxis; innate immune response; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; neurite development; cell structure disassembly during apoptosis; positive regulation of DNA binding

48-Strip-Wells

515 USD

96-Strip-Wells

695

5x96-Strip-Wells

2685

10x96-Strip-Wells

4850