ELISA/assay

Trypsin (TRY) ELISA Kit

MBS2021227

48-Strip-Wells

600 USD

ELISA Kit

Trypsin (TRY) ELISA Kit

Trypsin (TRY)

trypsin; Trypsin; Trypsin

TRY

CLOLEP_02546

A7VVD4_9FIRM

Porcine

556

This assay has high sensitivity and excellent specificity for detection of trypsin. No significant cross-reactivity or interference between trypsin and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Porcine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 1.56-100ng/mL. The standard curve concentrations used for the ELISA's were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.12ng/mL, 1.56ng/mL. Sensitivity: Typically less than 0.62ng/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level trypsin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level trypsin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of trypsin in porcine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to trypsin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to trypsin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain trypsin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of trypsin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

156867562

EDO60934.1

58,344 Da

48-Strip-Wells

600 USD

96-Strip-Wells

820

5x96-Strip-Wells

3205

10x96-Strip-Wells

5765