Caspase 14 (CASP14) ELISA Kit | MyBioSource MBS2021215 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Caspase 14 (CASP14) ELISA Kit

catalog :

MBS2021215

quantity :

24-Strip-Wells

price :

255 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2021215

products type :

ELISA Kit

products full name :

Caspase 14 (CASP14) ELISA Kit

products short name :

[Caspase 14 (CASP14)]

products name syn :

[Caspase 14 Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 14]

other names :

[caspase-14; Caspase-14; caspase-14; caspase 14, apoptosis-related cysteine peptidase]

products gene name :

[CASP14]

other gene names :

[CASP14; CASP14; CASP-14]

uniprot entry name :

CASPE_HUMAN

reactivity :

Human

sequence length :

242

specificity :

This assay has high sensitivity and excellent specificity for detection of CAS. No significant cross-reactivity or interference between CAS and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Tissue Homogenates, Cell Lysates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: 0.114ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CAS were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CAS were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products categories :

Apoptosis

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CAS in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CAS. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CAS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CAS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CAS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

6912286

ncbi acc num :

NP_036246.1

ncbi gb acc num :

NM_012114.2

uniprot acc num :

P31944

ncbi mol weight :

27,680 Da

ncbi summary :

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. [provided by RefSeq, Jul 2008]

uniprot summary :

CASP14: Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin. Complex of unprocessed caspase-14 and processed 19 kDa (p19) and 10 kDa (p10) subunits. In undifferentiated keratinocytes under postconfluency growth conditions (in vitro). Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum). Expressed in keratinocytes of hair shaft and sebaceous glands. In psoriatic skin only expressed at very low levels. Belongs to the peptidase C14A family. Protein type: EC 3.4.22.-; Protease. Chromosomal Location of Human Ortholog: 19p13.1. Cellular Component: cytoplasm; keratin filament; nucleus. Molecular Function: cysteine-type endopeptidase activity. Biological Process: epidermis development; DNA damage response, signal transduction resulting in induction of apoptosis; keratinization; proteolysis

size1 :

24-Strip-Wells

price1 :

255 USD

size2 :

48-Strip-Wells

price2 :

455

size3 :

96-Strip-Wells

price3 :

610

size4 :

5x96-Strip-Wells

price4 :

2470

size5 :

10x96-Strip-Wells

price5 :

4445

more info or order :

MyBioSource product webpage