ELISA/assay

Insulin Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit

MBS2021206

48-Strip-Wells

390 USD

ELISA Kit

Insulin Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit

Insulin Like Growth Factor Binding Protein 4 (IGFBP4)

BP4; HT29-IGFBP; IBP4

insulin-like growth factor-binding protein 4; Insulin-like growth factor-binding protein 4; insulin-like growth factor-binding protein 4; insulin-like growth factor binding protein 4

IGFBP4

IGFBP4; IGFBP4; BP-4; IBP4; IGFBP-4; HT29-IGFBP; IBP4; IBP-4; IGF-binding protein 4; IGFBP-4

IBP4_HUMAN

Human

258

This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 4 (IGFBP4). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 4 (IGFBP4) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates and other biological fluids. Assay Type: Sandwich. Detection Range: 3.13-200ng/mL. Sensitivity: Typically less than 1.28ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 4 (IGFBP4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 4 (IGFBP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 4 (IGFBP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 4 (IGFBP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein 4 (IGFBP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Insulin Like Growth Factor Binding Protein 4 (IGFBP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

62243290

NP_001543.2

NM_001552.2

P22692

17,635 Da

Metabolism Of Proteins Pathway (106230); Myometrial Relaxation And Contraction Pathways (198759); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908); Wnt Signaling Network Pathway (137962)

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]

IGFBP4: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q21.2. Cellular Component: extracellular space; extracellular region. Molecular Function: insulin-like growth factor I binding; insulin-like growth factor II binding. Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; cell proliferation; regulation of insulin-like growth factor receptor signaling pathway; cellular protein metabolic process; positive regulation of MAPKKK cascade; regulation of cell growth; inflammatory response; signal transduction; DNA metabolic process; skeletal development

48-Strip-Wells

390 USD

96-Strip-Wells

520

5x96-Strip-Wells

1940

10x96-Strip-Wells

3460