ELISA/assay

Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

MBS2021098

24-Strip-Wells

225 USD

ELISA Kit

Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

[Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)]

[EPO; CLGI; EPA; HCI; Erythroid Potentiating Activity; Collagenase Inhibitor; Metalloproteinase inhibitor 1; Fibroblast collagenase inhibitor]

[tissue inhibitor of metalloproteinase 1, partial; Metalloproteinase inhibitor 1; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; Tissue inhibitor of metalloproteinases 1; TIMP-1]

[TIMP1]

[TIMP1; TIMP1; EPA; EPO; HCI; CLGI; TIMP; CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1]

TIMP1_HUMAN

Human

40

This assay has high sensitivity and excellent specificity for detection of Tissue Inhibitors Of Metalloproteinase 1 (TIMP1). No significant cross-reactivity or interference between Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Standard Curve/Testing Data

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 0.156-10ng/mL. Sensitivity: < 0.059ng/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Tumor immunity; Infection immunity; Cardiovascular biology; Hepatology; Hormone metabolism

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Inhibitors Of Metalloproteinase 1 (TIMP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tissue Inhibitors Of Metalloproteinase 1 (TIMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tissue Inhibitors Of Metalloproteinase 1 (TIMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

994731

AAA75558.1

P01033

23,171 Da

Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); HIF-1 Signaling Pathway (695200); Hemostasis Pathway (106028); IL6-mediated Signaling Events Pathway (137932); Matrix Metalloproteinases Pathway (198900); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]

TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: Xp11.3-p11.23. Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region; basement membrane. Molecular Function: metalloendopeptidase inhibitor activity; protein binding; growth factor activity; protease binding; metal ion binding; cytokine activity. Biological Process: response to peptide hormone stimulus; platelet activation; extracellular matrix organization and biogenesis; negative regulation of metalloenzyme activity; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; blood coagulation; aging; negative regulation of apoptosis

24-Strip-Wells

225 USD

48-Strip-Wells

375

96-Strip-Wells

505

5x96-Strip-Wells

2000

10x96-Strip-Wells

3600