ELISA/assay

Clara Cell Protein 16 (CC16) ELISA Kit

MBS2021046

24-Strip-Wells

255 USD

ELISA Kit

Clara Cell Protein 16 (CC16) ELISA Kit

[Clara Cell Protein 16 (CC16)]

[SCGB1A1; CC10; CCSP; UGB; Uteroglobin; Urinary Protein 1; Clara Cell Secretory Protein; Secretoglobin Family 1A Member 1; Clara-Cell Specific 10-kD Protein]

[CC16]

Human

This assay has high sensitivity and excellent specificity for detection of Clara Cell Protein 16 (CC16). No significant cross-reactivity or interference between Clara Cell Protein 16 (CC16) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Lung lavage fluid, Cell culture supernates and Other Biological Fluids. Detection Range: 0.156-10ng/mL. Sensitivity: < 0.067ng/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Clara Cell Protein 16 (CC16) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Clara Cell Protein 16 (CC16) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Infection immunity; Cardiovascular biology; Pulmonology

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Clara Cell Protein 16 (CC16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Clara Cell Protein 16 (CC16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Clara Cell Protein 16 (CC16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Clara Cell Protein 16 (CC16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

24-Strip-Wells

255 USD

48-Strip-Wells

455

96-Strip-Wells

610

5x96-Strip-Wells

2470

10x96-Strip-Wells

4445